目的：探讨电针针刺太阳穴对形觉剥夺性近视(FDM)小鼠的眼参数和睫状体组织中连环蛋白β1(β-catenin)、整合素β1(integrin β1)表达的影响。

方法：选取3周龄健康C57BL/6J小鼠48只，随机分为正常(NC)组、FDM组、假针(sham)组及电针针刺太阳穴(Taiyang)组4组，每组12只小鼠。FDM组、sham组、Taiyang组小鼠右眼均配戴半透明自制眼罩诱导近视； 同时Taiyang组在太阳穴给予电针刺激； sham组使用细木棒模拟针刺操作(不刺入皮肤)； 正常组不做任何处理。利用红外验光仪、光学相干断层扫描(OCT)检测造模前、造模4 wk小鼠的屈光度和眼轴长度； 利用实时荧光定量PCR(qPCR)、蛋白免疫印记(WB)检测造模4 wk小鼠睫状体组织中β-catenin、integrin β1的表达情况。

结果：造模4 wk后，与NC组相比，FDM组、sham组屈光度均显著降低(均P<0.05)，眼轴长度均显著增加(均P<0.05)，β-catenin、integrin β1的表达均增加； 与FDM组和sham组相比，Taiyang组的屈光度显著增加(均P<0.05)，眼轴长度显著缩短(均P<0.05)，β-catenin、integrin β1的表达降低。

结论：电针针刺太阳穴能有效控制FDM小鼠近视的进展，其机制可能与睫状体组织β-catenin、integrin β1的表达水平相关。

METHODS:A total of 48 3-week-old healthy C57BL/6J mice were randomly divided into 4 groups: normal control(NC), FDM group, sham acupuncture(sham), and electroacupuncture at Taiyang acupoint(Taiyang), with 12 mice in each group. Mice in the FDM, sham, and Taiyang groups, wore translucent custom-made eye masks on the right eye to induce myopia. The Taiyang group received electroacupuncture stimulation at the Taiyang acupoint, while the sham group underwent non-penetrating stimulation with a blunt wooden stick. No intervention was performed on the NC group. Refraction and axial length were measured by infrared autorefractor and optical coherence tomography(OCT)before modeling and at 4 wk after modeling. The expression levels of β-catenin and integrin β1 in the ciliary body of mice at 4 wk after modeling were detected using quantitative real-time PCR(qPCR)and Western blotting(WB).

RESULTS:After modeling for 4 wk, compared with the NC group, the FDM and sham groups showed significantly decreased refractive power(both P<0.05), elongated axial length(both P<0.05), and increased β-catenin and integrin β1 expression. Compared with the FDM and sham groups, the Taiyang group showed significantly increased refractive power(both P<0.05), shortened axial length(both P<0.05), and decreased β-catenin and integrin β1 expression.

CONCLUSION:Electroacupuncture stimulation at the Taiyang acupoint effectively delayed the progression of myopia in FDM mice, and this effect may be partially mediated through modulating the expression of β-catenin and integrin β1 in the ciliary body.