蛋白组学在干眼研究中的应用进展

Application progress of proteomics in dry eye research

摘要:

干眼的病理机制复杂，涉及免疫、炎症等多种通路，需要整体研究方案从全局进行把控。各类组学技术可以从整体和全局的高度阐明生物体复杂的生理病理状态，提供更加全面的生物信息。质谱技术可以灵敏检测出泪液样本中的蛋白含量变化，为干眼的蛋白组学研究提供了便捷条件。目前蛋白组学在干眼类型鉴别、严重程度分级、疗效评价等方面都显示出应用价值，并且与代谢组学、微生物组学联用可以更加全面地阐释干眼发病机制。未来蛋白组学有望为干眼的精准诊疗提供更加有力的支持，并在靶向治疗中发挥优势。

Abstract:

The complex pathological mechanism of dry eye involves multiple pathways, such as immunity and inflammation, and requires an integral research program to control the whole picture. Various histological techniques can elucidate the complex physio-pathological state of organisms from a holistic and global perspective, thus providing more comprehensive biological information. Mass spectrometry can sensitively detect the changes of protein content in tear samples, providing convenience for proteomics research of dry eye. At present, proteomics has demonstrated its application in the identification of dry eye types, severity grading, and therapeutic effect evaluation. In addition, proteomics combined with metabolomics and microbiomics can more comprehensively explain the pathogenesis of dry eye. In the future, proteomics is expected to provide more powerful support for the precise diagnosis and treatment of dry eye, taking an advantage in targeted therapy.