[关键词]
[摘要]
目的:观察糖尿病合并年龄相关性白内障患者晶状体上皮细胞(LECs)中色素上皮衍生因子(PEDF)、血管内皮细胞生长因子(VEGF)的表达水平,探讨糖尿病合并年龄相关性白内障的发病机制。
方法:回顾性研究。收集2020-08/2021-04在蚌埠医学院第一附属医院眼科就诊的年龄相关性白内障和2型糖尿病合并年龄相关性白内障患者各30例。所有患者白内障超声乳化术中收取术眼晶状体中央区5.5~6.0mm直径的前囊膜标本,采用蛋白免疫印迹法(Western-blot)检测LECs中PEDF、VEGF蛋白表达水平。实时荧光定量PCR(qRT-PCR)方法检测PEDF、VEGF mRNA的相对表达量。
结果:两组患者LECs中均存在PEDF、VEGF的表达,2型糖尿病合并年龄相关性白内障组患者VEGF mRNA相对表达量为1.364±0.062,高于年龄相关性白内障组的1.000±0.0(P<0.01),PEDF mRNA的相对表达量为0.398±0.053,明显低于年龄相关性白内障组的1.000±0.0(P<0.001)。2型糖尿病合并年龄相关性白内障组患者LECs中VEGF和PEDF蛋白表达量为2.053±0.026、0.579±0.045,年龄相关性白内障组为1.680±0.064、1.058±0.007(均P<0.01)。
结论:2型糖尿病合并年龄相关性白内障患者LECs中PEDF和VEGF表达水平发生变化,可能与糖尿病患者白内障的发生和发展有关。
[Key word]
[Abstract]
AIM: To observe the expression levels of pigment epithelium-derived factor(PEDF)and vascular endothelial growth factor(VEGF)in lens epithelial cells(LECs)of diabetic patients with age-related cataract, and explore the pathogenesis of diabetic with age-related cataract.
METHODS: Retrospectively study. A total of 30 patients with age-related cataract(ARC group)and 30 patients with type 2 diabetes mellitus combined with age-related cataract(DC group)who treated at the Department of Ophthalmology of the First Affiliated Hospital of Bengbu Medical College from August 2020 to April 2021 were collected. Anterior capsule specimens with a diameter of 5.5-6.0mm in the central region of the intraoperative eye were collected during phacoemulsification. The protein expression levels of PEDF and VEGF in LECs were detected by Western-blot. Quantitative real-time PCR(qRT-PCR)was used to detect the relative mRNA expression levels of PEDF and VEGF.
RESULTS:PEDF and VEGF were expressed in LECs of both groups, and the relative expression of VEGF mRNA in DC group was 1.364±0.062, which was higher than that of ARC group(1.000±0.0; P<0.01). The relative mRNA expression level of PEDF was 0.398±0.053, which was significantly lower than 1.000±0.0 in the ARC group(P<0.001). The expression of VEGF protein in LECs was 2.053±0.026 in DC group and 1.680±0.064 in ARC group, respectively. Meanwhile, the expression of PEDF protein was 0.579±0.045 in DC group and 1.058±0.007 in ARC group(all P<0.01).
CONCLUSION:The changes of PEDF and VEGF expression levels in LECs may be related to the occurrence and development of cataract in diabetic patients.
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[基金项目]
安徽省大学生创新创业计划项目(No.S202010367029); 蚌埠医学院科技项目(No.2020byzd141)