目的：探索模拟失重环境与视网膜损伤的关系。

方法：采用尾吊小鼠试验模拟失重，应用光镜和透射电镜观察视网膜组织形态学变化； 应用免疫组织化学和Western blot检测与分析相关的分子变化。

结果：光镜和透射电镜显示：与对照组相比，尾吊组小鼠视网膜神经节层和内核层细胞中均可见更多死亡细胞，相关通路中的TNF-α和caspase-3蛋白表达上调。

结论：在模拟失重环境下小鼠视网膜会产生明显的病理损伤。

METHODS: Hanging-tail mice tests were used to simulate weightlessness. Light microscopy, and transmission electron microscopic examinations of the retinal tissue structure were used to observe morphological changes. Immunohistochemistry and Western blot analyses were performed to detect the molecular changes associated with the observations.

RESULT: Light microscopy and transmission electron microscopy demonstrated that more dead cells were detected in the ganglion layer and inner nuclear layer cells. TNF-α and caspase-3 protein expression in the retinas of simulated microgravity groups were up-regulated compared with the ground-based control group.

CONCLUSION: The results demonstrated that simulated microgravity produced severe pathological damage in the retinas of mice.