方法：选取青紫蓝兔48只经制作眼球穿通伤及玻璃体腔内注射0.3mL富含血小板血浆的方法制备TPVR动物模型，随机分为4组(n=12)，其中对照组玻璃体腔注入0.1mL生理盐水； TA组玻璃体腔注入0.1mL(1mg/mL)曲安奈德； ART组玻璃体腔注入0.1mL(20μg/mL)青蒿琥酯； LU组玻璃体腔注入0.1mL(10μg/mL)木犀草素。术后1、2、3、4wk通过眼底照相和眼部B超观察玻璃体及视网膜增生情况，术后28d采用Western Blot法检测各组兔眼玻璃体液中α-SMA和VIM蛋白表达水平，并经视网膜HE染色观察各组视网膜组织结构情况。

结果：术后28d，TA组、ART组和LU组兔眼TPVR分级均显著低于对照组(P<0.05)，且TA组兔眼TPVR分级均显著低于ART组和LU组(P<0.05)。术后28d，TA组、ART组和LU组兔眼玻璃体液中α-SMA和VIM蛋白的表达水平均显著低于对照组(P<0.01)。HE染色结果显示，对照组兔眼视网膜各层排列紊乱，严重扭曲或局部断裂，各层结构不清晰，前膜明显增厚，视网膜明显脱离； LU组兔眼视网膜各层排列轻微扭曲，视网膜前可见炎性渗出，视网膜浅层脱离； ART组兔眼视网膜结构清晰，轻度水肿，可见浅层脱离； TA组兔眼视网膜各层结构清晰，排列尚整齐，局部可见视网膜褶皱，无视网膜脱离。

结论：玻璃体腔内注射曲安奈德、青蒿琥酯及木犀草素均对TPVR具有防治作用，其中曲安奈德效果最明显。

METHODS: Forty-eight cyanotic blue rabbits were selected to prepare TPVR animal models by making a penetrating eye injury and intravitreal injection of 0.3mL platelet-rich plasma, and were randomly divided into four groups(n=12), in which the vitreous cavity of the control group was injected with 0.1mL saline; The vitreous cavity of the TA group was injected with 0.1mL(1mg/mL)triamcinolone acetonide; The vitreous cavity of the ART group was injected with 0.1mL(20μg/mL)artesunate; 0.1mL(10μg/mL)luteolin was injected into the vitreous cavity of the LU group. The vitreous and retinal proliferation were observed by fundus photography and ocular ultrasound at 1, 2, 3 and 4wk postoperatively. The expression levels of α-SMA and VIM protein in the vitreous fluid of each group of rabbit eyes were detected by Western Blot at 28d postoperatively, and the retinal tissue structure of each group was observed by retinal HE staining.

RESULTS: At 28d postoperatively, the TPVR grading of rabbit eyes in the TA, ART and LU groups were significantly lower than that in the control group(P<0.05), and the TPVR grading of rabbit eyes in the TA group was significantly lower than that in the ART and LU groups(P<0.05). The expression levels of α-SMA and VIM proteins in the vitreous fluid of the rabbit eyes in the TA, ART and LU groups were significantly lower than those in the control group at 28d after surgery(P<0.01). The results of HE staining showed that the arrangement of retinal layers in rabbit eyesin the control group were disordered, severely distorted or locally broken, the structure of each layer were unclear, the anterior membrane was obviously thickened, and the retina was obviously detached; The arrangement of retinal layersin rabbit eyes in the LU group were slightly distorted, inflammatory exudation was visible in front of the retina, and the retina was superficially detached; The structure of retina in rabbit eyes in the ART group were clear, with mild edema and superficial detachment; The structure of retinal layers in rabbit eyes in the TA group were clear, the arrangement was still neat, the retinal folds were locally visible, and there was no retinal detachment.

CONCLUSION: Intravitreal injection of triamcinolone acetonide, artesunate and luteolin were all effective in preventing and treating traumatic TPVR, among which triamcinolone acetonide has the most obvious effect.