目的：研究电子烟对小鼠视网膜组织及超微结构的影响以及可能的相关机制。

方法：将18只8周龄雄性c57BL小鼠随机分为对照组(6只)，0mg尼古丁组(6只)和12mg尼古丁组(6只)，分别用HE染色观察视网膜各层结构的改变，在透射电子显微镜下观察视网膜色素上皮(RPE)细胞超微结构的改变，通过免疫荧光染色观察视网膜中Tuj1、8-OHdG的表达情况。

结果：与对照组相比，实验组(0mg和12mg尼古丁组)视网膜全层、视神经纤维层和内丛状层厚度均显著减少(P<0.01)，但实验组间均无明显差异(P>0.05)。RPE细胞顶部仅见零星微绒毛，残存微绒毛长度变短。实验组中节细胞层、视神经纤维层和内丛状层可观察到Tuj1表达减少，但是神经节细胞数目无显著变化(P>0.05)。0mg和12mg尼古丁组中节细胞层、内核层内观察到8-OHdG表达增加。

结论：电子烟可对小鼠视网膜造成损伤，其损伤可能是由氧化应激反应造成的。

METHODS: Totally 18 male c57BL mice aged 8-week-old were divided into control group(n=6), 0mg nicotine group(n=6)and 12mg nicotine group(n=6). The histological and ultrastructural changes of retina were evaluated by hematoxylin and eosin(HE)staining and transmission electron microscope(TEM), respectively. Additionally, the expression of Tuj1 and 8-OHdG was examined using immunofluorescent staining.

RESULTS: In comparison with control group, the thickness of whole retina, nerve fiber layer(NFL)and inner plexiform layer(IPL)was significantly decreased in experimental groups(0mg and 12mg nicotine group)(P<0.01), but no significant difference was observed between 0mg and 12mg nicotine group(P>0.05). The dramatically reduced microvilli of RPE cells were also observed in experimental groups using TEM. Furthermore, residual microvilli were shortened. The expression of Tuj1 was decreased in ganglion cell layer(GCL), NFL and IPL, but no significant changes in the number of retinal ganglion cells were shown among three groups(P>0.05). In addition, the increased expression of 8-OHdG was observed in GCL and inner nuclear layer(INL)in experimental groups.

CONCLUSION: E-cigarette can lead to the retinal damages in mice, which might be due to oxidative stress.