炎症及动脉硬化相关因子与视网膜静脉阻塞的相关性

Correlation between inflammation and arteriosclerosis related factors and retinal vein occlusion

摘要:

目的：研究视网膜静脉阻塞(RVO)患者血清白介素6(IL-6)、脂联素(ADPN)、Apelin、超敏C反应蛋白(hs-CRP)、血管内皮生长因子(VEGF)水平变化情况，从分子角度探究其与RVO的相关性。

方法：收集中央型视网膜静脉阻塞(CRVO)35例，分支型视网膜静脉阻塞(BRVO)37例为试验组1、2，年龄相关性白内障患者32例为对照组，记录其发病时间、视力、心脑血管疾病病史及OCT示黄斑水肿高度，酶联免疫吸附法检测其外周血血清中与炎症及动脉硬化紧密相关因子—IL-6、ADPN、Apelin、hs-CRP、VEGF的表达情况。

结果：BRVO组、CRVO组Apelin值分别为6.69(4.25，10.52)、7.12(3.78，8.58)ng/mL，均高于对照组1.19(0.74，1.49)ng/mL(P<0.05)。BRVO组、CRVO组ADPN值分别为8.06(4.67，10.81)、9.74(4.10，11.67)μg/mL。BRVO组、CRVO组IL-6值分别为35.89(17.63，37.50)、37.16(11.52，42.80)pg/mL。BRVO组、CRVO组hs-CRP值分别为161.10(54.51，164.01)、206.93(51.47，331.29)μg/mL。BRVO组、CRVO组VEGF值分别为158.25(82.24，230.41)、174.14(76.04，243.98)pg/mL。该四项因子试验组与对照组比较均无差异(P>0.05)。RVO组视力及黄斑水肿高度与上述血清因子水平未见明显相关。

结论：RVO患者Apelin增高，且Apelin可能为RVO发生的相关危险因素。ADPN、IL-6、hs-CRP、VEGF在RVO急性期未见特异性表达。

Abstract:

AIM: To study the changes of serum IL-6, ADPN, Apelin, hs-CRP and VEGF levels in patients with RVO, and to explore their association with the occurrence and development of RVO from a molecular perspective.

METHODS:Totally 72 cases of RVO, including 35 cases of central retinal vein occlusion(CRVO)and 37 cases of branch retinal vein occlusion(BRVO). They were divided into groups 1 and 2. 32 senile cataract patients were collected as control group. The time of onset, visual acuity, history of cardio cerebral vascular disease and Macular edema thickness were recorded. Enzyme linked immunosorbent assay was used to detect the expression of IL-6, ADPN, Apelin, hs-CRP and VEGF in blood serum, which were closely related to inflammation and arteriosclerosis.

RESULTS: The mean value of Apelin in group BRVO was 6.69(4.25, 10.52)ng/mL, and CRVO group was 7.12(3.78, 8.58)ng/mL, and the two groups were higher than those in control group 1.19(0.74, 1.49)ng/mL(P<0.05). The mean value of ADPN in group BRVO was 8.06(4.67, 10.81)μg/mL, while in group CRVO was 9.74(4.10, 11.67)μg/mL. The mean value of IL-6 in group BRVO was 35.89(17.63, 37.50)pg/mL, while in group CRVO was 37.16(11.52, 42.80))pg/mL. The mean value of hs-CRP in group BRVO was 161.10(54.51, 164.01)μg/mL. The mean value of VEGF in group BRVO was 158.25(82.24, 230.41)pg/mL, while in group CRVO was 174.14(76.04,243.98)pg/ml(P>0.05). There was no significant difference between the experimental group and the control group.

CONCLUSION:By comparing the levels of serum IL-6, ADPN, Apelin, hs-CRP and VEGF in 72 RVO patients and 32 senile cataract patients, Apelin was found to be higher than that in the control group. Apelin may be one of the related risk factors for RVO. The expression of ADPN, IL-6, hs-CRP, VEGF, RVO in acute stage no specificity.