目的：了解复发性霰粒肿患者人体蠕形螨的感染情况及引起霰粒肿复发的可能相关因素。

方法：收集2019-01/05于我院眼表和角膜病科门诊就诊确诊“霰粒肿”的患者，A组32眼为复发性霰粒肿组，B组30眼为初发性霰粒肿组，C组35眼为无眼病的正常对照组。生物光学显微镜观察蠕形螨睫毛根部阳性感染率，活体激光扫描共聚焦显微镜检查睫毛根部、睑板腺管开口蠕形螨感染情况。

结果：生物光学显微镜观察情况：A组患者睫毛的蠕形螨检出率为78%，显著高于B组(57%)和C组(34%)。共聚焦显微镜观察情况：A组患者睫毛的蠕形螨检出率为88%，显著高于B组(67%)和C组(37%)； A组患者睑板腺开口的蠕形螨检出率为69%，显著高于B组(23%)和C组(14%)。

结论：复发性霰粒肿患者蠕形螨感染率明显较高，蠕形螨感染可能是顽固性霰粒肿致病原因之一。

METHODS: Patients diagnosed with "chalazion" in our ocular surface and cornea department from January 2019 to May 2019 were collected. 32 eyes in group A were patients with recurrent chalazion, 30 eyes in group B were patients with primary chalazion, and 35 eyes in group C were patients without eye disease. The positive infection rate of the roots of Demodex lashes was observed by biological optical microscopy. The infection of Demodex in the roots of the eyelashes and the opening of the meibomian glands was observed by vivo laser scanning confocal microscopy.

RESULTS: Observations by biological optical microscope: the detection rate of Demodex in the eyelashes of group A was 78%, which was significantly higher than that of group B(57%)and group C(34%). Observations by confocal microscopy: the detection rate of Demodex in the eyelashes of group A was 88%, which was significantly higher than that of group B(67%)and group C(37%). The detection rate of Demodex in the meibomian gland opening of group A was 69%, which was significantly higher than that of group B(23%)and group C(14%).

CONCLUSION: The rate of Demodex infection in patients with recurrent chalazion is obviously higher. Demodex infection may be one of the pathogenic reasons for recurrent chalazion.