目的：探究羊膜泪小管支架对手术阉割雄兔干眼症的影响。

方法：将36只成年雄性兔分组，其中A组行假阉割手术，B组假植入泪小管支架，C组植入泪小管支架。分别于植入前和植入后2、4、6wk行角膜荧光素染色(FL)和泪液分泌试验(SⅠt)检查，共聚焦显微镜观察角膜神经变化。

结果：A组角膜光滑、清晰，FL染色不明显； B组角膜粗糙，角膜上皮见点染色斑点； C组角膜较假手术组更为光滑、清晰，无明显点状染色。各组植入后2、4、6wk时FL染色评分和SⅠt比较，差异有统计学意义(均P<0.05)。共聚焦显微镜下可见A组为相对直的神经丛，量多，清晰可见； B组角膜上皮细胞下神经可见弯曲，数量有所减少； C组角膜上皮下神经相对直，与正常雄兔角膜上皮下神经相比，数量略有减少。

结论：羊膜泪小管支架可显著改善阉割去势雄兔干眼症的症状和体征，具有治疗作用。

METHODS: Thirty-six adult male rabbits were divided into two groups. Group A was treated with false castration, group B was pseudo-implantation group, and group C was implanted with lacrimal canal. Fluorescein(FL)staining and scoring and SⅠt examination, and confocal microscopy were performed at 2, 4, 6wk after implantation.

RESULTS: The cornea of group A was smooth and clear, and the FL staining was not obvious. In group B, the cornea was rough, and the corneal epithelium was spotted with stained spots. The corneal surface of group C was smoother and more transparent than that of sham operation group and the punctate staining was not obviously. The FL staining scores and SⅠt examination were significant(P<0.05)at 2, 4, and 6wk after implantation. FL staining and scoring and SⅠt, tear protein determination, confocal microscopy changes in corneal nerve. Confocal microscopy showed that group A was a relatively straight plexus, the amount was large, clearly visible. In the group B, the inferior nerve of the corneal epithelial cells was visible and the number was reduced. The corneal epithelial nerve in group C was relatively straight, and the number was slightly reduced compared with the normal male rabbit corneal epithelial nerve.

CONCLUSION: The amniotic lacrimal duct stent can significantly improve the symptoms and signs of castrated castrated male rabbit dry eye syndrome, and has a therapeutic effect.