目的：探究血管内皮生长因子(VEGF)及趋化因子受体3(CCR3)在翼状胬肉组织中的表达及意义。

方法：纳入行翼状胬肉切除联合自体结膜瓣移植术的患者18例18眼，术中取翼状胬肉组织； 行巩膜扣带环扎术的孔源性视网膜脱离患者14例14眼，术中取鼻侧球结膜组织。采用HE染色法观察翼状胬肉和正常结膜的组织学差异，采用免疫化学法分析VEGF和CCR3的表达水平，采用qRT-PCR法检测VEGF和CCR3 mRNA的表达水平。

结果：与正常结膜组织相比，翼状胬肉组织上皮层明显增厚，基质层组织排列紊乱，且VEGF(0.69±0.0875 vs 0.05±0.0024)和CCR3(0.45±0.0248 vs 0.03±0.0074)表达水平显著升高(均P<0.01)。翼状胬肉组织中VEGF mRNA表达水平约为正常结膜组织的12倍(12.33±2.84 vs 1.00±0.08)，CCR3 mRNA的表达水平约为正常结膜组织的160倍(159.60±34.15 vs 1.00±0.09)。

结论：翼状胬肉组织中VEGF和CCR3表达明显升高，提示二者可能参与翼状胬肉的发生，促进疾病的发展。

METHODS: The expression of VEGF and CCR3 were detected in 18 patients(18 eyes)with pterygium and compared with the normal conjunctiva epithelium in other 14 cases(14 eyes). The histological differences between the two groups were compared by hematoxylin and eosin(HE)staining. The expression of VEGF and CCR3 in pterygium and normal conjunctival tissues were analyzed by immunofluorescence and immunohistochemical. The mRNA expression of VEGF and CCR3 were assessed by Real-time PCR.

RESULTS: Compared with normal conjunctival tissue, the epithelial layer of pterygium was obvious thickening, the stromal layer was more disordered, and the expression levels of VEGF(0.69±0.0875 vs 0.05±0.0024)and CCR3(0.45±0.0248 vs 0.03±0.0074)were significantly elevated(all P<0.01). The expression level of VEGF mRNA in pterygium was about 12 times that of normal conjunctival tissue(12.33±2.84 vs 1.00±0.08), and the expression level of CCR3 mRNA was about 160 times as many as conjunctival tissue(159.60±34.15 vs 1.00±0.09).

CONCLUSION: The expression of VEGF and CCR3 in pterygium was significantly increased, suggesting that they may be involved in the occurrence of pterygium and promote the development of the disease.