息肉状脉络膜血管病变患者房水中IL-8及其受体的表达

Expression of IL-8 and its receptor in aqueous humor of patients with polypoid choroidal vasculopathy

摘要:

目的：探究息肉状脉络膜血管病变(PCV)患者房水中IL-8及其受体1、2(CXCR1、CXCR2)的水平，并探讨其临床意义。

方法：选取2016-04/2018-03本院收治的PCV患者67例作为PCV组，另选取白内障患者50例作为对照组。采用实时荧光定量PCR法检测两组患者房水中IL-8、CXCR1、CXCR2 mRNA表达量，采用国际标准视力表测量所有受试者BCVA，OCT检测CMT，创建受试者工作特征曲线(ROC)分析IL-8、CXCR1、CXCR2对PCV的诊断价值； 采用Logistic多因素回归分析评估PCV发生的危险因素。

结果：与对照组相比，PCV组患者房水中IL-8、CXCR1、CXCR2 mRNA表达升高，BCVA降低，CMT升高(均P<0.05)。PCV组患者房水中IL-8、CXCR1、CXCR2水平与BCVA(LogMAR)呈正相关(r=0.438、0.346、0.385，均P<0.05)，与CMT呈正相关(r=0.378、0.606、0.357，均P<0.05)。IL-8、CXCR1、CXCR2、BCVA、CMT是PCV发生的危险因素。IL-8、CXCR1、CXCR2诊断PCV的ROC曲线下面积(AUC)分别为0.882、0.860、0.812。

结论：PCV患者房水中IL-8、CXCR1、CXCR2表达均升高，其不仅与患者BCVA和CMT相关，且与PCV的发生有关，可能成为PCV的潜在预测指标。

Abstract:

AIM: To explore the levels of interleukin-8, interleukin-8 receptor 1, 2(CXCR1, CXCR2)in aqueous humor of polypoid choroidal vasculopathy(PCV)and their clinical significance.

METHODS: Totally 67 patients with PCV in our hospital were selected as PCV group, and another 50 patients with cataract were selected as control group, fluorescence quantitative PCR(QPCR)was used to detect the expression of IL-8, CXCR1 and CXCR2 mRNAs in aqueous humor of two groups, the best corrected visual acuity(BCVA)of all subjects were measured by the international standard visual acuity chart, optical coherence tomography(OCT)was used to detect foveal retinal thickness(CMT), the receiver operating characteristic curve(ROC)was created to analyze the predictive values of IL-8, CXCR1 and CXCR2 in the occurrence of PCV. Multivariate Logistic regression analysis was used to assess the risk factors for PCV.

RESULTS: Compared with the control group, the expression of IL-8, CXCR1 and CXCR2 mRNA increased in group PCV. BCVA in PCV group decreased and CMT increased(P<0.05). IL-8, CXCR1 and CXCR2 were significantly positively correlated with BCVA(r=0.438, 0.346, 0.385, all P<0.05), IL-8, CXCR1 and CXCR2 were positively correlated with CMT(r=0.378, 0.606, 0.357, all P<0.05). Logistic multivariate regression analysis showed that IL-8, CXCR1, CXCR2, BCVA and CMT were the risk factors of PCV. The AUC of IL-8, CXCR1 and CXCR2 for PCV diagnosis was 0.882, 0.860 and 0.812.

CONCLUSION: The expression of IL-8, CXCR1 and CXCR2 in PCV aqueous humor were all increased, which was not only related to BCVA and CMT in patients, but also related to the occurrence of PCV, and it may become a potential predictor of PCV.