BMP-6与新生血管性年龄相关性黄斑变性的相关性

Correlation analysis of BMP-6 and age-related neovascular macular degeneration

摘要:

目的：探讨新生血管性年龄相关性黄斑变性(nARMD)患者房水中骨形态发生蛋白6(BMP-6)、白细胞介素-6(IL-6)及血管内皮生长因子(VEGF)的变化及其与黄斑区视网膜厚度的相关性。

方法：收集2015-06/2017-03于我院眼科就诊的nARMD患者34例35眼作为nARMD组，选取同期于我院住院行白内障手术的年龄相关性白内障患者20例20眼作为对照组。采用光学相干断层扫描(OCT)测量两组患者黄斑区1mm范围内的平均视网膜厚度(CSMT)。通过ELISA法检测房水中BMP-6的浓度，流式微球技术(CBA)测量IL-6和VEGF的浓度。

结果：nARMD组患者房水中BMP-6水平(35.29±4.27pg/mL)明显低于对照组(62.04±2.78pg/mL)，而VEGF水平(93.13±47.25pg/mL)明显高于对照组(69.21±13.40pg/mL)，差异均有统计学意义(P<0.05)，两组患者房水中IL-6的水平差异无统计学意义(P>0.05)。Spearman相关性分析表明，nARMD组患者房水中BMP-6含量与CSMT呈负相关(r_s=-0.409，P=0.015)。

结论：nARMD患眼房水中BMP-6浓度下降，而VEGF浓度升高，BMP-6含量与CSMT呈负相关，其可能参与nARMD的发病过程。

Abstract:

AIM: To investigate the correlations between aqueous concentrations of bone morphogenetic protein 6(BMP-6), interleukin 6(IL-6), and vascular endothelial growth factor(VEGF)and the parameters of macular edema acquired by optical coherence tomography(OCT)in patients with neovascular age-related macular degeneration(nARMD).

METHODS: Totally 35 eyes from 34 patients with nARMD were studied. Twenty eyes from 20 patients with no retinal pathology who underwent cataract surgery served as controls. The central subfield macular thickness(CSMT)of nARMD within 1 mm was measured with OCT. Aqueous humor samples were collected just before intravitreal ranibizumab injection in 35 eyes with nARMD and before cataract surgery in 20 control eyes. Concentration of BMP-6 were determined by ELISA. IL-6, VEGF were measured by cytometric bead array(CBA).

RESULTS: The level of BMP-6 in aqueous humor of patients with nARMD(35.29±4.27pg/mL)was significantly lower than that of control eyes(62.04±2.78pg/mL, P<0.05), while VEGF level was 93.13±47.25pg/mL, significantly higher than that of the control group 69.21±13.40pg/mL(P<0.05). The level of IL-6 was not significantly different between the two groups. BMP-6 was negatively correlated with CSMT(r=-0.409, P=0.015).

CONCLUSION: Aqueous humor concentrations of BMP-6 are decreased in eyes with nARMD. Aqueous humor concentrations of VEGF are elevated in eyes with nARMD. BMP-6 could be associated with the pathogenesis of nARMD.