目的：观察不同浓度密蒙花滴眼液对去势雄兔泪腺细胞炎症因子TNF-α、IL-1β的影响，探讨不同浓度密蒙花滴眼液对去势雄兔干眼症模型的疗效。

方法：将密蒙花原药材制备成低、中、高3种浓度滴眼液。将42只健康成年新西兰长耳白兔(雄性)，随机分为A：空白组； B：模型组； C：低浓度密蒙花滴眼液组； D：中浓度密蒙花滴眼液组； E：高浓度密蒙花滴眼液组； F：安慰剂滴眼液组； G：睾酮组等7组，每组6只。除A组外其余6组实验用兔行双侧睾丸及附睾切除术。从术后第3天开始C、D、E、F组分别予相应滴眼液滴双眼。各组实验用兔，分别于术前和术后第4wk各进行一次Schirmer I试验(SIT)和泪膜破裂时间(BUT)测定。采用免疫组化法对泪腺细胞炎症因子TNF-α和IL-1β的表达进行检测。

结果：1)SIT与BUT值比较：术后C组与D、E、G组比较，有统计学意义(P<0.01)； D、E、G组间相比较，无统计学意义(P>0.05)； 2)术后各组炎症因子TNF-α、IL-1β比较：C组与D、E、G组比较，有统计学意义(P<0.05)； D、E、G组间相比较，无统计学意义(P>0.05)。

结论：1)密蒙花滴眼液具有与雄激素相似的抑制细胞炎症因子表达的作用，但其作用弱于雄激素； 2)中、高浓度密蒙花滴眼液对TNF-α和IL-1β的抑制作用要强于低浓度密蒙花滴眼液，但中、高浓度密蒙花滴眼液的抑制作用差别不明显。

METHODS:Forty-two healthy adult New Zealand rabbits were randomly divided into 7 groups, 6 rabbits each group. Group A: blank group; Group B: model group; Group C: low concentrations BOED group; Group D: medium concentrations BOED; Group E: high concentrations BOED; Group F: the placebo group; Group G: testosterone groups. All rabbits of groups B to G were cut off bilateral testis and epididymis except group A. The rabbits of groups C, D, E and F were dropped relevant eye drops except groups A and B. All rabbits were tested for Schimer I test(SIT)and break-up time(BUT)before operation and 4wk after operation. Expressions of inflammatory factors of TNF-α, IL-1β from lacrimal gland cells were checked in immunohistochemical staining method.

RESULTS:1)Comparison of SIT and BUT: Compared with the groups D, E and G, group C had statistical significance(P<0.01). Compared with groups D and E, G groups, there was no significant difference among those group after operation(P>0.05); 2)Comparison of inflammatory factors of TNF-α, IL-1β after operation: Compared with value of the average optical density of TNF-α and IL-1β of group C, those of groups D, E and G were obviously different after operation(P<0.05). Compared D with E, G groups, there was no significant difference between those group after operation(P>0.05).

CONCLUSION: 1)Compared with androgen, BOED has the similar effect of depressing the expression of inflammatory factors. But its effect is slightly weaker than the androgen; 2)The depressing effect of medium and high concentration BOED are better than low concentration. Compared with medium and high concentration of BOED, there was no significant difference between two groups.