目的：探讨长期配戴硬性角膜接触镜对泪膜稳定性的影响，观察硬性角膜接触镜长期配戴的安全性。

方法：本组观察对象为2014-10/2016-01于我院进行配戴硬性角膜接触镜矫正的近视患者60例120眼以及无硬性角膜接触镜配戴史的低度近视患者30例60眼，将白天配戴RGP的患者26例52眼设为白天配戴组，只在夜间配戴OK镜的患者34例68眼设为夜间配戴组，无硬性角膜接触镜配戴史的低度近视患者30例60眼设为对照组。戴镜前、戴镜1、6mo，1、2a 进行泪液分泌试验(Schirmer I test，SⅠt)、泪膜羊齿状试验以及泪膜破裂时间(break-up time ，BUT)检测。

结果：白天配戴组与夜间配戴组戴镜后各时间点BUT、SⅠt均较戴镜前降低，且均显著低于对照组，差异有统计学意义(P<0.05)，戴镜后6mo，1、2a白天配戴组BUT、SⅠt显著低于夜间配戴组，差异有统计学意义(P<0.05)； 三组患者配镜前、配镜1、6mo后泪液羊齿状试验分级比较，差异无统计学意义(P>0.05)； 配镜1、2a白天配戴组与夜间配戴组≥Ⅲ级眼数显著高于对照组，差异有统计学意义(P<0.05)； 白天配戴组与夜间配戴组组间比较差异无统计学意义(P>0.05)。

结论：长期配戴硬性角膜接触镜可使泪液分泌量降低，泪膜稳定性下降。

METHODS: Totally 60 myopia patients(120 eyes)wearing RGP in our hospital from October 2014 to January 2016 and 30 low myopia patients(60 eyes)without a history of wearing RGP were selected. There were 26 patients(52 eyes)wearing RGP at daytime were included into daytime wearing group; 34 patients(68 eyes)only wearing RGP at night were set as the night wearing group. And patients without wearing RGP were set as control group. Schirmer Ⅰ test(SⅠt), tear film fern experiments, and break-up time(BUT)of tear film were performed before wearing RGP, wearing for 1mo, wearing for 6mo, wearing for 1a, wearing for 2a, respectively.

RESULTS: After wearing RGP, the BUT and SⅠt of daytime wearing group and night wearing group significantly decreased, and were significantly lower than those of the control group at the time points, the difference was statistically significant(P<0.05). After wearing for 6mo, 1a and 2a, the BUT and SⅠt of daytime wearing group was significantly lower than that of the night wearing group, the statistic found significant difference(P<0.05). The three groups had no statistically significant differences in tear fern classification experiment before wearing RGP and wearing for 1 and 6mo(P>0.05). The number of the eyes ≥ Ⅲ in daytime wearing group and night wearing group was significantly higher than that of the control group after wearing for 1 and 2a, the difference was statistically significant(P<0.05), but the difference between daytime wearing group and night wearing group found no significant difference(P>0.05).

CONCLUSION: Wearing RGP can result in reducing tear secretion and decreasing the stability of tear film.