目的：研究氪激光联合Q-开关Nd：YAG激光行激光虹膜切除术后一过性眼压升高的发病机制。

方法：选取由本院动物实验中心提供的健康家兔42只84眼，其中雌兔18只，雄兔24只； 平均质量2.24±0.31kg； 随机分为6组，每组7只14眼，其中A、B、C、D、E组分别为激光虹膜切除术后20min，2、6、18、24h组； F组为正常对照组。观察各组术前及术后眼压和房水内丙二醛(malondialdehyde，MDA)、一氧化氮(nitric oxide，NO)、超氧化物歧化酶(superoxide dismutase，SOD)、6-酮-前列腺素F_1α(6-酮-PGF1α)和一氧化氮合酶(nitric oxide synthase，NOS)含量的变化。

结果：手术前各组眼压、房水内NO、NOS、SOD、MDA、6-酮-PGF1α含量对比差异均无统计学意义(P>0.05)。术后6h内眼压升高，A、B、C组分别与F组比较，差异均有统计学意义(P<0.05)，术后6h以后呈下降趋势，D、E组分别与F组比较，差异均无统计学意义(P>0.05)。术后房水内NO、NOS、SOD含量呈下降趋势，A、B、C组分别与F组比较，差异均有统计学意义(P<0.05)，术后6h以后慢慢恢复，D、E组分别与F组比较，差异均无统计学意义(P>0.05)。术后房水内MDA和6-酮-PGF1α含量呈上升趋势，A、B、C组分别与F组比较，差异均有统计学意义(P<0.05)，术后6h以后慢慢恢复，D、E组分别与F组比较，差异均无统计学意义(P>0.05)。

结论：激光虹膜切除术后一过性眼压升高与术后房水内MDA、6-酮-PGF1α含量升高和SOD、NO降低相关。

METHODS: Totally 42 healthy rabbits(84 eyes)provided by the Animal Experimental Center of our hospital were selected, including 18 female rabbits, 24 male rabbits, average weight 2.24±0.31kg, and they were randomly divided into 6 groups, 7 rats in each group(14 eyes). We observed the change of intraocular pressure after laser iridectomy surgery at 20min, 2, 6, 18, 24h and the nitric oxide(NO), malondialdehyde(MDA), superoxide dismutase(SOD), 6-keto-prostaglandin(6-keto-PGF1α)and nitric oxide synthase(NOS)content in aqueous.

RESULTS: There was no significant difference in intraocular pressure, NO, NOS, SOD, MAD and 6-keto- PGF1α before operation(P>0.05). The intraocular pressure increased after operation, and the difference was statistically significant(P<0.05)at 20min, 2 and 6h after operation, and decreased at 18h after operation, 24h after operation(P>0.05). The levels of NO, NOS and SOD in the aqueous humor of the two groups decreased 20min, 2 and 6h after the operation(P<0.05), while increased after 6h, increased more at 18 and 24h.The difference with control group was no more significant(P>0.05). The levels of MDA and 6-keto-prostaglandin in the aqueous humor increased after the operation, and the difference was statistically significant at 20min, 2 and 6h after operation(P<0.05), while decreased at 18 and 24h and the difference with control group was not significant(P>0.05).

CONCLUSION: The increase of transient intraocular pressure after laser iridectomy may relate to the increase of malondialdehyde, 6-keto-prostaglandin content and the decrease of superoxide dismutase and nitric oxide in the aqueous humor after operation.