[关键词]
[摘要]
目的：研究氯化锂(lithium chloride，LiCl)对体外培养的人眼Tenon囊成纤维细胞(human Tenon's capsule fibroblasts，HTFs)转化生长因子β(transforming growth factor-β，TGF-β)及结缔组织生长因子(connective tissue growth factor，CTGF)表达的影响并探讨其作用机制。
方法：体外培养HTFs并通过vimentin免疫荧光染色技术及细胞形态特征观察鉴定细胞； 将HTFs分为实验组(80mmol/L LiCl处理48h)和对照组(未用药)。用实时荧光定量PCR(Real-time fluorescent quantitative polymerase chain reaction， Real time-qPCR)检测两组细胞内TGF-β及CTGF基因的表达，Western blot检测两组细胞内TGF-β及CTGF蛋白的表达。
结果：体外培养的HTFs能够表达TGF-β及CTGF。与对照组比较，实验组中TGF-β、CTGF基因表达下降，差异均具有统计学意义(t=20.042、14.995，P<0.05)； 与对照组比较，实验组中TGF-β、CTGF蛋白表达下降，差异均具有统计学意义(t=46.058、12.452， P<0.05)。
结论：体外培养的HTFs在基因和蛋白水平表达TGF-β及CTGF，LiCl能够抑制该过程，其可能通过该机制抑制HTFs增殖，此研究为LiCl用于青光眼滤过术后瘢痕化调控提供了实验依据。
[Key word]
[Abstract]
AIM:To research the effects of lithium chloride on transforming growth factor beta(TGF-β)and connective tissue growth factor(CTGF)in cultured human Tenon capsule fibroblasts(HTFs)and explore its mechanism.
METHODS: HTFs were cultured and identified by vimentin staining with immunofluorescence and the morphological characteristics. The experimental group was processed 48h with LiCl in concentration of 80mmol/L, the control group without LiCl. The mRNA expression of TGF-β and CTGF in two groups were analyzed with real-time fluorescent quantitative polymerase chain reaction(real time-qPCR)and the protein expression was detected with Western blot.
RESULTS:The cultured HTFs expressed TGF-β and CTGF. The mRNA expression of TGF-β and CTGF significantly decreased compared with the control group(t=20.042, 14.995, P<0.05). the protein expression of TGF-β and CTGF also decreased significantly compared with the control group(t=46.058、12.452, P<0.05)
CONCLUSION: The cultured HTFs can express TGF-β and CTGF in mRNA and proteins' level. LiCl can reduce the expression of TGF-β and CTGF both in gene and proteins' level. LiCl has the potential to modulate wound healing for glaucoma filtration surgery.
[中图分类号]
[基金项目]
潍坊市科学技术发展计划项目(No.2015WS017)
