目的：探讨人羊膜匀浆提取液对角膜碱烧伤后新生血管形成过程中色素上皮衍生因子(PEDF)和血管内皮生长因子(VEGF)表达及角膜新生血管的影响。

方法：选取2015-06/2016-06在佛山爱尔眼科医院治疗的角膜碱烧伤患者32例37眼，随机分为A、B两组。其中A组17例19眼，采用40mg/L人羊膜匀浆提取液治疗，B组15例18眼，采用3g/L泼尼松龙滴眼液治疗。在治疗不同的时间点(1、4、7、14、21、28d)观察角膜新生血管的生长，同时检测新生血管形成过程中PEDF及VEGF的表达水平。

结果：A组患者在使用人羊膜匀浆提取液治疗后，PEDF表达水平显著高于B组，差异有统计学意义(P=0.001)，在治疗28d后，PEDF表达水平达到了0.721±0.314，而B组患者PEDF表达水平仅有0.538±0.253，两组患者PEDF表达水平间的差异具有统计学意义(P<0.05)； A组VEGF表达水平在不同的时间点检测时均低于B组，在治疗28d后，A组患者VEGF表达水平为0.152±0.020，B组患者VEGF表达水平为0.302±0.031，两组患者VEGF表达水平间的差异具有统计学意义(P<0.05)； A组患者角膜新生血管数量显著低于B组患者，差异有统计学意义(P<0.05)。

结论：人羊膜匀浆提取液可以促进患者角膜碱烧伤后新生血管形成过程中PEDF表达，抑制VEGF的表达和角膜新生血管的增殖。

AIM: To analyze the effect of human amniotic homogenate extract on corneal neovascularization after corneal alkali burn in the process of pigment epithelium derived factor(PEDF)and vascular endothelial growth factor(VEGF)expression and the effect of corneal neovascularization.

METHODS: Totally 32 patients with corneal alkali burn were selected from June 2015 to June 2016 in Foshan, and were randomly divided into Group A and Group B, with a total of 37 eyes. Group A of 17 cases, with a total of 19 eyes, were treated with 40mg/L human amniotic homogenate extract; Group B(n=15), and 18 eyes, treated with 3g/L prednisolone eye drops. In the treatment of 1, 4, 7, 14, 21 and 28d at different time points, we observed the growth of corneal neovascularization, and detected the expression of PEDF and VEGF during angiogenesis.

RESULTS: Group A of patients in the use of human amniotic homogenate extract after the treatment, the expression level of PEDF was significantly higher than that in Group B(P=0.001), after 28d treatment, the expression level of PEDF reached 0.721±0.314. While patients in Group B the expression level of PEDF was only 0.538±0.253. Two groups had significant difference between the expression level of PEDF(P<0.05). The expression level of VEGF in Group A was lower than in Group B at different time points in the test. After the treatment of 28d patients in the Group A, the expression level of VEGF was 0.152±0.020, in Group B the expression level of VEGF was0.302±0.031. Two groups of patients with VEGF expression level between the differences were statistically significant(P<0.05). The patients number in Group A with corneal neovascularization was significantly lower than that in Group B, the difference was statistically significant(P<0.05).

CONCLUSION: Human amniotic homogenate extract can increase the expression of PEDF in corneal neovascularization after corneal alkali burn, inhibit the expression of VEGF and the proliferation of corneal neovascularization.