方法：采用氪激光诱导棕色挪威(brown Norway，BN)大鼠建立实验性CNV模型。24只雄性BN大鼠，随机分为正常对照组(6只)与模型组(18只)。模型组行659nm氪激光视网膜光凝，功率360mW，曝光时间0.05s，光斑直径50μm。光凝后7、14、21d行荧光素钠和吲哚菁绿眼底血管造影，观察CNV形成率及渗漏光斑平均光密度值(average optical density，AOD)变化。处死大鼠，获得眼球标本，观察CNV的组织病理学变化和CD105因子的表达。

结果：光凝后7d，CNV开始形成，14d和21d达到高峰。光凝后7、14、21d的CNV形成率分别为77.08%、85.42%、89.58%。光凝后7～21d，荧光素渗漏AOD值逐渐增高(P<0.05)，7d与14d比较差异有统计学意义(P<0.05)，14d与21d比较差异无统计学意义(P>0.05)。造模后7～21d，CD105因子表达先升高后下降，7d与14d比较AOD值差异有统计学变化(P<0.05)，14d与21d比较AOD值差异无统计学变化(P>0.05)。

结论：CD105免疫组织化学与眼底血管造影对CNV变化规律的研究结果具有高度一致性，CD105免疫组织化学评价CNV具有准确、易操作的优点。

METHODS: Twenty-four male Brown Norway(BN)rats were randomly divided into the control group(6 rats)and the experimental group(18 rats). The CNV were induced by 659nm krypton laser. And the power was 360mW, exposure time was 0.05s, spot diameter was 50μm. The formation rate of CNV and the average optical density(AOD)of leakage flare were observed by fluorescein fundus angiography(FFA)and indocyanine green angiography(ICGA)on the seventh day, the fourteenth day and the twenty-first day after laser photocoagulation. The histopathology changes of CNV and the AOD of CD105 were observed by eyeball exemplar.

RESULTS: The CNV appeared on the seventh day after laser photocoagulation, and reached the peak on the fourteenth day and twenty-first day after laser photocoagulation. The formation rate of CNV were 77.08%, 85.42%, 89.58%, at 7, 14 and 21d after laser photocoagulation. From 7 to 21d after laser photocoagulation, the AOD of leakage flare increased gradually(P<0.05). There had significant differences between 7 days' outcome and 14 days' outcome(P<0.05), and had no significant differences between 14 days' outcome and 21 days' outcome(P>0.05). From 7 to 21d after laser photocoagulation, the expression of CD105 increased gradually, and then decreased gradually, and from 14 to 21d, there had significant differences between 7 days' outcome and 14 days' outcome of AOD(P<0.05), and had no significant differences between 14 days' outcome and 21 days' outcome(P>0.05).

CONCLUSION: Immunohistochemical outcomes of CD105 are highly consistent with fundus angiography outcomes of CNV changing regularity.