方法：收集武汉大学人民医院眼科2011-01/2015-12住院患者送检标本中分离培养出的阳性菌株，运用WHONET 5.6软件进行数据分析。

结果：眼科住院患者的送检样本共4 486例，共分离培养出病原微生物736株，其中革兰阳性菌510株，革兰阴性菌107株，真菌119株。革兰阳性菌中最常见的为表皮葡萄球菌(247株)，其次为棒状杆菌(153株)； 革兰阴性菌最多见的是铜绿假单胞菌(39株)； 真菌最常见的是镰刀菌(77株)，其次是曲霉菌(31株)。主要葡萄球菌对利福平、甲氧苄啶、利奈唑胺、万古霉素和替考拉宁，肺炎链球菌对左氧氟沙星、利奈唑胺、万古霉素和替考拉宁，非发酵菌对头孢哌酮/舒巴坦和多黏菌素，肠杆菌科细菌对亚胺培南、美罗培南和左氧氟沙星敏感性均为100%； 耐甲氧西林葡萄球菌、青霉素耐药肺炎链球菌的检出率分别为37.5%、40.0%。

结论：加强病原菌耐药性监测，及时了解眼科感染病原菌的流行特点及耐药趋势，对临床合理应用抗生素进行抗感染治疗从而减少耐药菌的产生及医院制定感染控制措施有着重要的意义。

METHODS:In this study, we collected positive strains isolated from the hospitalized patients in Renmin Hospital of Wuhan University from January 2011 to December 2015. Data were analyzed with WHONET 5.6 software.

RESULTS: A total of 4 486 cases of ophthalmic inpatients were included in the study, 736 strains of pathogenic microorganisms were isolated, including 510 gram-positive bacteria, 107 gram-negative bacteria and 119 fungi. The most common type of gram positive bacteria as the epidermis staphylococcus(247 strains), followed by coryne bacterium(153 strains). The most common type of gram negative bacteria for pseudomonas aeruginosa(39 strains). The most common type of fungi for sickle bacterium(77 strains), followed by aspergillus strain(31 strains). The staphylococcus was sensitive to rifampicin, trimethoprim, linezolid, vancomycin and teicoplanin. The Streptococcus pneumoniae was sensitive to levofloxacin, linezolid, vancomycin and teicoplanin. The drug sensitive rates of non-fermenters to cefoperazone/sulbactam and colistin, enterobacteriaceae to imipenem, meropenem and levofloxacin were all 100%. The detection rates of methicillin-resistant staphylococci and penicillin-resistant Streptococcus pneumoniae were 37.5% and 40.0%, respectively.

CONCLUSION:We should strengthen the monitoring of pathogen resistance, to keep abreast of the epidemic characteristics of ophthalmic infection pathogens and drug resistance trends, which are of great significance for the rational application of antibiotics in clinical anti-infective treatment, reducing the emergence of drug-resistant bacteria and hospital infection control measures.