方法：RPE细胞传代培养，分为阴性对照组：以正常培养液培养； 氧化损伤组：100μmol/L的H₂O₂作用12h； 槲皮素低浓度组：100μmol/L槲皮素孵育24h后，加入H₂O₂作用12h； 槲皮素高浓度组：500μmol/L槲皮素孵育24h后，加入H₂O₂作用12h。MTT比色法检测细胞活力，流式细胞仪检测细胞凋亡率，Hochest33258染色观察凋亡细胞形态，比色法检测细胞中过氧化氢酶(catalase，CAT)、超氧化物歧化酶(superoxide dismutase，SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase，GSH-Px)活性。

结果：槲皮素能明显抑制H₂O₂诱导的RPE细胞活力的下降，用不同浓度槲皮素处理后，RPE细胞活性分别提高到79.67%±4.98%和83.00%±3.60%，与氧化损伤组(48.93%±3.39%)比较，差异具有统计学意义(P<0.05)； 经不同浓度槲皮素处理后，RPE细胞凋亡率分别下降至23.23%±3.29%和16.23%±1.94%，与氧化损伤组(38.03%±4.76%)比较，差异具有统计学意义(P<0.05)； 此外，槲皮素还能增加细胞中CAT、SOD、GSH-Px活性，与氧化损伤组比较，差异均具有统计学意义(P<0.05)。

结论：槲皮素通过改善细胞中抗氧化酶活性有效抑制了H₂O₂对RPE细胞的损伤，从而为其用于治疗RPE细胞损伤提供可靠的实验依据。

METHODS: RPE cells were subculture, and they were divided into negative control group: cultured with normal culture medium; oxidative injury group: 100 μmol/L H₂O₂ treated for 12h; quercetin low dose group: 100 μmol/L quercetin incubated for 24h then treated with 100 μmol/L H₂O₂ for 12h; and quercetin high dose group: 100 μmol/L quercetin incubated for 24h then treated with 100 μmol/L H₂O₂ for 12h. Cell viability were tested by MTT colorimetric detection, apoptosis rate was detected by flow cytometry, apoptotic cell morphology was observed by Hochest33258 staining, expression of catalase(CAT), superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were tested by colorimetric detection.

RESULTS: Quercetin inhibited H₂O₂-induced cell viability decreased in RPE cells, after treated with different concentrations of quercetin, RPE cells activity increased to(79.67±4.98)% and(83.00±3.60)%, which had statistical significance difference compared with oxidative damage group(48.93±3.39)%(P<0.05). After treated with different concentrations of quercetin, the apoptosis rate of RPE cells decreased to(23.23±3.29)% and(16.23±1.94)%, respectively, which had statistical significance difference compared with oxidative damage group(38.03±4.76)%(P<0.05). In addition, quercetin also increased the expression of CAT、SOD、GSH-Px in RPE cells, which had statistical significance difference compared with oxidative damage group.

CONCLUSION:Quercetin effectively inhibited H₂O₂-induced RPE cells damage by improving cell antioxidant enzyme activity, which provide reliable experimental basis for the treatment of injuries in RPE cells.