方法：健康雄性SD大鼠25只，随机选取5只作为正常对照组； 其余20只采用一次性腹腔注射链脲佐菌素(STZ，60mg/kg)诱导糖尿病大鼠模型，建模成功(血糖>16.7mmol/L)后应用随机数字表法分为糖尿病对照组、0.01%丹酚酸B凝胶治疗组、0.05%丹酚酸B凝胶治疗组、0.1%丹酚酸B凝胶治疗组，丹酚酸B治疗组和糖尿病对照组每天分别给予自制相应浓度的丹酚酸B凝胶及空白凝胶点眼，每次1滴，每日3次； 检测大鼠的血糖和体质量，观察角膜知觉和透明度，每周1次； 造模成功后的第12wk取材，观察角膜组织结构改变，以及大鼠眼组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性情况。

结果：所有大鼠血糖处在高水平(>16.7mmol/L)，体质量早期上升后期有明显下降。在糖尿病对照组，角膜的知觉和透明度降低，各丹酚酸B凝胶治疗组与糖尿病对照组相比较，角膜知觉明显改善，各丹酚酸B治疗组角膜保持透明度与正常组无明显差异，角膜组织中的MDA含量明显减少，角膜组织中SOD的活性明显增加(P<0.05)。

结论：丹酚酸B可以减缓糖尿病大鼠角膜病变，其机制可能与丹酚酸B能清除超氧阴离子的抗氧化应激作用有关。

METHODS: Twenty-five Sprague-Dawley male rats were taken as experimental subjects. Random 5 of them were used as control group. The remaining 20 were induced to be diabetic mellitus by a single intraperitoneal injection of streptozotocin(STZ)and divided into 4 groups randomly: diabetes control group, 0.01% Salvianolic acid B treated group, 0.05% Salvianolic acid B treated group and 0.1% Salvianolic acid B treated group. In the Salvianolic acid B treated groups, the corresponding concentration of Salvianolic acid B eye drops were used 3 times a day until the animal was sacrificed, and then detected rats blood glucose and body weight, observed sensation and transparency of the cornea once a week. After the building completion, all rats in each group were sacrificed in 12^ndwk, and then observed the corneal tissue structure change, and in rat serum and tissue of malondialdehyde(MDA)content and activity of superoxide dismutase(SOD).

RESULTS: The blood glucose of all rats was in high level(>16.7mmol/L), early rise and later fall in weight. In the diabetic control group, the corneal sensation and transparency of the cornea were decreased. The content of MDA in the corneal tissue was significantly increased. The activity of SOD in the corneal tissue was significantly decreased(P<0.05). Through the course of disease, the above change was obvious day by day. Compared with the diabetic control group, the sensation and transparency of the cornea were improved significantly. The content of MDA in the corneal tissue was significantly decreased. The activity of SOD in the corneal tissue was significantly increased(P<0.01).

CONCLUSION: Salvianolic acid B can reduce the corneal lesions of diabetic rats, the mechanism may be related to Salvianolic acid B, remove superoxide anion, protect cells from damage of resisting oxidative stress.