方法：将72例120眼睑板腺功能障碍患者分为轻中度组36例60眼和重度组36例60眼，采用酶联免疫吸附试验检测睑板腺分泌物和泪液中PGE₂的含量。正常人30例60眼设为对照组，只检测泪液中PGE₂含量。

结果：泪液中PGE₂含量在三组间差异有显著统计学意义(F=100.26，P<0.01)，重度组泪液PGE₂含量高于轻中度组(t=5.789，P<0.01)和正常组(t=7.468，P<0.01)，轻中度组泪液PGE₂含量与正常组差异无统计学意义(t=0.923，P>0.05)； 睑板腺分泌物PGE₂含量在两组间差异有统计学意义，重度组高于轻中度组(F=151.76，P<0.01)； 重度组泪液与睑板腺分泌物中PGE₂含量呈正相关(r=0.71，P<0.01)； 轻中度组泪液与睑板腺分泌物PGE₂含量呈正相关(r=0.30，P<0.05)。

结论：睑板腺分泌物和泪液PGE₂含量对判断睑板腺功能障碍的严重程度和指导临床合理用药具有重要作用。

METHODS:Seventy-two patients with MGD were divided into mild to moderate group(36 patients with 60 eyes)and severe group(36 patients with 60 eyes).The content of PGE₂ in Meibomian gland secretions and tear were tested by ELISA. Thirty healthy adults(60 eyes)were chosen as the control group which were only tested for PGE₂ in tear.

RESULTS:The differences of the content of PGE₂ in tear in the three groups were significant(F=100.26,P<0.01),and that in the severe group was higher than that in the mild to moderate group(t=5.789,P<0.01)and the control group(t=7.468,P<0.01). There was no significant difference of the content of PGE₂ in tear between the mild to moderate group and control group(t=0.923,P>0.05). The differences of the content of PGE₂ in Meibomian gland secretions in three groups were significant and that in the severe group was much higher than that in the mild to moderate group(F=151.76,P<0.01).The content of PGE₂ in Meibomian gland secretions were positive correlated to the content of PGE₂ in tears in severe group(r=0.71,P<0.01); the content of PGE₂ in Meibomian gland secretions was positive correlated to the content of PGE₂ in tears in mild to moderate group(r=0.30,P<0.05).

CONCLUSION:The level of PGE₂ in Meibomian gland secretions and tears plays an important role in judging the severity of MGD and guiding clinical rational drug use.