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[摘要]
目的：探讨番茄红素对紫外线诱导的人晶状体上皮细胞(human lens epithelial cells，HLEC)氧化损伤的保护作用及可能机制。
方法：HLEC传代培养，分为阴性对照组、氧化损伤组、番茄红素低剂量组和番茄红素高剂量组，MTT比色法检测细胞存活率，电子显微镜观察细胞形态学改变，DCFH-DA荧光探针检测胞内ROS变化，分光光度计检测上清液中超氧化物歧化酶(superoxide dismutase，SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase，GSH)活性及丙二醛(malondialdehyde，MDA)的含量。
结果：番茄红素能明显抑制紫外线诱导的细胞活性的下降，减少紫外线所致HLEC内ROS的生成，引起HLEC内SOD和GSH-Px水平的升高及MDA水平的下降。
结论：番茄红素通过提高细胞内SOD和GSH-Px含量，降低细胞内MDA含量，发挥其较强的抗氧化作用，从而为其用于白内障防治提供可靠的实验依据。
[Key word]
[Abstract]
AIM:To investigate the protective effect and possible mechanisms of lycopene for oxidative damage induced by ultraviolet in cultured human lens epithelial cells(HLEC).
METHODS:HLEC was subcultured and divided into negative control group, oxidative injury group, lycopene low dose group and lycopene high dose group. Cell viability was assayed by MTT colorimetric. Cell morphological changes were detected by electron microscope. Reactive oxygen species(ROS)levels were detected with DCFH-DA fluorescent probe. Content of superoxide dismutase(SOD), glutathione peroxidase(GSH)and malondialdehyde(MDA)in supernatants were detected by spectrophotometer.
RESULTS:Lycopene could obviously inhibited UV-induced decline in cell activity, reduce UV-induced ROS generation within HLEC, cause SOD, GSH-Px levels increased and MDA levels decreased.
CONCLUSION:Lycopene plays its strong antioxidant role in increasing the intracellular SOD and GSH-Px content levels and decreasing MDA levels, which provide reliable experimental basis for prevent and treatment of cataracts.
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