[关键词]
[摘要]
目的：检测Vogt-小柳原田综合征(VKH)患者和正常对照人群血浆中miRNAs差异表达谱，探讨miRNAs在VKH发病中的作用，寻找疾病相关的血浆生物标记物。
方法：采集10例VKH患者和10例正常对照组的血液样本，提取血浆中的总RNA，先进行RNA逆转录合成cDNA，然后在96孔板中利用miRNA实时定量PCR芯片进行Real-time PCR扩增和溶解曲线分析。获得原始的Ct值，应用Exiqon GenEx qPCR analysis software分析原始数据获得差异表达miRNA，并对差异表达miRNA进行生物信息学分析。
结果：miRCURY LNATM Universal RT microRNA PCR芯片一共有179个人类来源的miRNAs，共筛选出20个差异表达的miRNAs，其中在VKH组中高表达者有12个，低表达者有8个。差异表达miRNAs参与多种信号通路。
结论：Vogt-小柳原田综合征患者血浆中存在多个microRNA异常表达，异常表达的miRNAs可能通过某种信号通路参与VKH的发病。
[Key word]
[Abstract]
AIM:To explore the differences of expression pattern of microRNA(miRNA)in plasma from Vogt-Koyanagi-Harada(VKH)syndrome patients and normal controls, searching for diseases-relating biomarkers.
METHODS:Blood samples from 10 cases of VKH patients and 10 cases of normal control were collected to extracted total RNA in plasma. The cDNA was synthesized by reverse transcription, and then underwent real time PCR in a 96 well plate from miRCURY LNATM Universal RT microRNA PCR panels.Original Ct value was obtained. The Exiqon GenEx qPCR analysis software was used to analyse the original data and the differential miRNAs was obtained and analyzed by bioinformatics.
RESULTS:The panels contained 179 miRNAs classes from human individuals. In comparison with the healthy controls,there were 20 differential miRNAs, in which 12 miRNAs were up regulated and 8 miRNA were down regulated(all P<0.05). These microRNAs participated in the regulation of various signaling pathways.
CONCLUSION:The VKH patients and healthy controls have significantly different expression profiles of microRNA.The differential expression of microRNA may be involved in the pathogenesis of VKH.
[中图分类号]
[基金项目]
北京市自然科学基金(No.7122042)
