方法：选择PDGFR-α shRNA/lip2 000比值1：1、1：2、1：3(其中PDGFR-α shRNA分别为2μg、3μg和4μg)制备复合物转染至人视网膜色素上皮细胞(human retinal pigment epithelium，HRPE)中，24h后分别取0.1mL注射到家兔玻璃体腔。选取健康成年有色家兔40只随机分为平衡盐溶液(balanced salt solution， BSS)组(N组)，含lipofectamine^TM2 000的HRPE细胞稀释液组(A组)，取最佳转染效率的1.0、1.5与2.0μmol/L含PDGF-α受体的shRNA及lipofectamine^TM2000的HRPE细胞稀释液组(B、C和D组)，每组8眼，右眼为实验眼。间接眼底镜观察PVR程度，免疫组织化学染色进行切片着色情况观察及组织病理学观察眼底改变。

结果：PDGFR-α shRNA/lip2 000比值1：2时，HRPE细胞最佳转染效率； B组、C组和D组PVR程度、组织病理学改变、免疫组织化学染色PDGFR-α浓度低于A组，D组比B组和C组更低。

结论：PDGF-α受体mRNA的RNA干扰对实验性PVR形成有抑制作用。

METHODS:Different concentrations of PDGFR-α shRNA were blended with lip2000,and the final scale of PDGFR-α shRNA/lip2 000 complex was 1:1, 1:2 and 1:3 respectively(the concentration of PDGFR-α shRNA was respectively 2μg, 3μg and 4μg).All the complexes were cultivated in human retinal pigment epithelium(HRPE)for 24h after transfection and then respectively injected 0.1mL to rabbit vitreous cavity.Forty healthy adult rabbits were seleceted in this study and randomly divided into colored balanced salt solution(BSS)group(N), comprising lipofectamine^TM2 000 HRPE cell dilution group(group A). The highest transduction efficiency of 1.0, 1.5 and 2.0μmol/L containing PDGFR-α receptors shRNA, lipofectamine^TM2 000 of HRPE cell dilution were selected(respectively group B, C and D)with 8 eyes each, the right eyes as the experimental eye. The extent of PVR was observed by indirect ophthalmoscope; and slice staining situation was observed by immunohistochemistry.The fundus changes were observed by histopathology.

RESULTS:The highest transduction efficiency of PDGFR-αshRNA/lip2 000 ratio was 1:2.The extent of PVR, the histopathology changes and the immunohistochemistry of PDGFR-α in group B,C and group D were significantly lower than that in group A, while group D was much lower than those of group B and C.

CONCLUSION:PDGFR-αRNA interference could inhibit the formation of experimental PVR.