方法：将新生C57BL/6J小鼠随机分为3组，分别为常氧对照组、生理盐水注射氧诱导视网膜病变(oxygen-induced retinopathy，OIR)组和脂联素注射OIR组。将后两组小鼠于生后第7d(P7)至P12置于体积分数75%±2%高氧氧箱中以诱导OIR模型。脂联素注射OIR模型组在P7～P15每天接受腹腔注射重组鼠脂联素蛋白(3.0μg/g)，生理盐水注射OIR组则于上述相同时间点注射同等剂量的生理盐水。三组小鼠均于P17时取右眼行视网膜铺片和Lectin染色，观察视网膜中央无血管区及病理性新生血管的情况； 取左眼行视网膜切片和HE染色，观察视网膜组织病理学变化； 应用Western-blot测定左眼视网膜组织中iNOS、nNOS、eNOS的表达； 取右眼视网膜组织定量检测ROS/RNS含量以及NO水平。

结果：脂联素注射OIR组小鼠视网膜中央无血管区面积较生理盐水注射OIR组明显减少(t=7.304，P<0.01)，病理性新生血管数目明显减少(t=2.654，P<0.01)； 脂联素注射OIR组小鼠视网膜组织ROS含量较生理盐水注射组明显降低(t=13.349，P<0.01)； 脂联素注射OIR组小鼠视网膜组织NO含量明显高于生理盐水注射组(t=3.023，P<0.01)，iNOS表达明显低于生理盐水注射组(t=5.112，P<0.01)，eNOS表达明显高于生理盐水注射组(t=7.421，P<0.01)，nNOS的表达无统计学差异(t=1.074，P>0.01)。

结论：脂联素可以通过激活内源性eNOS促进生理性NO生成，同时又能抑制ROS/RNS的产生，在OIR进程中发挥视网膜血管的保护作用。

METHODS: Neonatal C57BL/6J mice were divided randomly into three groups: normoxic control group, physiological saline injection of OIR group and adiponectin injection of OIR group. The mice of the latter two groups were exposed to 75%±2% oxygen from 7d(P7)～P12 to induced OIR. Recombinant APN(rAPN)was injected intraperitoneally(i.p., 3.0μg/g)in a mice model of OIR from P7～P15. Another set of mice model of OIR were received a similar treatment with physiological saline. All eyes were collected at P17. The right eyes were whole mounted and stained with Lectin to observe central retinal avascular area and the growth of pathological neovascularization; The left eyes were performed histopathological cross sections stained with HE to analyzed the histopathological changes in the retina. The eyes were enucleated to assess the levels of reactive oxygen species(ROS)and NO. The protein expression of iNOS, nNOS, eNOS were detected by Western-blot.

RESULTS: The central retinal avascular area, neovascular area were markedly decreased after the APN injection compared with physiological saline injection of OIR group(t=7.304, P<0.01; t=2.654, P<0.01). Compared with physiological saline injection of OIR group, the levels of ROS were lower(t=13.349, P<0.01), the levels of NO were higher(t=3.023, P<0.01), the expression of iNOS were decreased(t=5.112, P<0.01), the expression of eNOS were decreased(t=7.421, P<0.01). nNOS expression had no significant difference(t=1.074, P>0.01).

CONCLUSION:The realtus demonstrate that APN can promote physiological NO by acting endogenous eNOS, while suppress ROS/RNS generation and play a protective role in retinal vessels in OIR process.