方法：制备SD大鼠补肾养血明目方含药血清及空白血清，通过CCK-8检测不同浓度(2.5%，5%，10%，20%，40%)血清对细胞活性的影响，预先加入1.25%，2.5%，5%等不同浓度的含药血清处理24h后，将终浓度为75μmol/L的丙烯醛加入对数生长期的ARPE-19细胞中处理24h， CCK-8法检测含药血清对细胞活力的影响，DAPI染色观察细胞核形态。

结果：CCK-8结果示：低浓度(2.5%，5%)血清对细胞活性影响不大(P>0.05)，高浓度(10%，20%，40%)血清降低细胞活性(P<0.05)，补肾养血明目方含药血清中、高剂量组细胞活性较空白血清组有统计学意义(P<0.05)；

结论：补肾养血明目方对丙烯醛诱导的ARPE-19细胞损伤有保护作用。

METHODS: SD rats serum containing the prescription of reinforcing kidney, nourishing blood, improving eyesight and the content of distilled water in serum were prepared. The effects of the prescription and distilled water in serum at different concentration(2.5%, 5%, 10%, 20% and 40%)on cell vitality was observed by cell counting kit(CCK-8)assay. the logarithmic phase of ARPE-19 cells were pretreated by different concentrations(1.25%, 2.5% and 5%)of the prescription serum and distilled water in serum for 24h. Then it was treated with 75μmol/L acrolein for 24h. Cell vitality was observed by CCK-8 assay. The change of cell nucleus was detected by DAPI staining.

RESULTS: 2.5% and 5% serum had no effect on cell viability(P>0.05), while 10%, 20%, 40% serum could inhibit cell viability(P<0.05). CCK-8 results showed that 2.5% and 5% the prescription of reinforcing kidney, nourishing blood, improving eyesight serum was better than distilled water in serum(P<0.05).

CONCLUSION: The prescription of reinforcing kidney, nourishing blood, improving eyesight has the protective effect on ARPE-19 cell damage induced by acrolein.