BMSCs联合ChABC对视网膜变性大鼠光感受器细胞凋亡的影响

Bone mesenchymal stem cells and chondroitinaseABC on photoreceptor apoptosis in degenerated retina of rats

摘要:

目的：研究骨髓间充质干细胞(bone mesenchymal stem cells，BMSCs)联合硫酸软骨素酶(chondroitinaseABC，ChABC)行视网膜下腔注射对碘酸钠诱导的视网膜变性大鼠光感受器细胞凋亡的影响。

方法：选取40只SD大鼠行腹腔注射碘酸钠(NaIO₃，30g/L，100mg/kg)造视网膜变性模型，分为A组不干预组，B组BMSCs注射组，C组BMSCs+ChABC注射组，D组PBS注射组。造模后28d将ChABC处理或未处理的BMSCs注射入大鼠视网膜下腔，对照组注射PBS液，21d后处死大鼠并取出眼球，行视网膜HE染色、视网膜细胞凋亡及免疫组化检测。

结果：B组凋亡率、外核层细胞数与A组、D组比较，差异均有统计学意义(P<0.05)。C组凋亡率、外核层细胞数与A组、D组比较，差异均有统计学意义(P<0.05)。B组凋亡率、外核层细胞数与C组相比，差异无统计学意义(P>0.05)。免疫组化显示BMSCs在眼内表达GFAP抗原。

结论：BMSCs联合ChABC行视网膜下腔注射可缓解视网膜变性大鼠光感受器细胞的凋亡，延缓细胞数目的减少，从而保护视网膜光感受器细胞。

Abstract:

AIM: To study the effect of bone mesenchymal stem cells(BMSCs)and chondroitinaseABC(ChABC)on photoreceptor apoptosis in the retina of sodium iodate-induced rats.

METHODS: Forty Sprague Dawley rats(SD rats)were intraperitoneally injected with NaIO₃ (30g/L, 100mg/kg)to establish the retinal degeneration models(postnatal 28d). These rats were devided into 4 groups. Group A was not injected, group B was injected with BMSCs, group C was injected with BMSCs and ChABC, and group D was injected with phosphate buffer saline(PBS). After 28d, subretinal injection were applied. Hematoxyln-eosinstaining(HE), tunel and immunohistochemistry were performed at 21d after subretinal injection.

RESULTS: Photoreceptor number and photoreceptor apoptosis rate of B and C groups were more than those of A and D groups, and there was significant difference statistically(P<0.05). Photoreceptor number and photoreceptor apoptosis rate of group B were compared with those of group C, and there was no statistical significance between B and C groups(P>0.05). Glial fibrillary acidic protein(GFAP)was expressed by BMSCs after intraocular injection.

CONCLUSION: BMSCs and ChABC injected into subretinal space may alleviate photoreceptor apoptosis so as to protect retinal photoreceptor cells in degenerated rats.