方法：选取疼痛性大泡性角膜病(PBK)患者 156例156眼(均为单眼)，行角膜层间灼烙联合羊膜移植术，术后随访2～3mo，观察患者术眼疼痛症状、角膜上皮水泡、异物感、术后并发症等指标。

结果：疼痛症状全部消失，角膜上皮不再出现水泡130例(83.3%)； 患者剧烈眼疼消失，但偶有异物感等不适，角膜上皮偶有小水泡24例(15.4%)； 术后2wk内角膜基质发生溶解，再行结膜瓣覆盖后治愈2例(1.3%)。

结论：角膜层间灼烙联合羊膜移植术能解除98.7%的PBK患者的痛苦且手术操作简单，值得提倡。

METHODS: Randomly selected 156 cases(156 eyes)with PBK who underwent surgery of intralamellar cryolysis of cornea with amniotic membrane transplantation. Followed up with 2～3mo, the symptoms of eye pain, corneal epithelial blisters, foreign body sensation and postoperative complications were observed.

RESULTS: Pain symptoms disappeared in all patients, and corneal epithelial blisters disappeared in 130 cases(83.3%). All agonizing pain symptoms disappeared, but patients had occasional foreign-body sensation, occasional corneal epithelial blisters in 24 cases(15.4%). Two weeks after surgery, corneal stroma dissolved, 2 cases(1.3%)of them were cured by conjunctival flap cover.

CONCLUSION: The operation of intralamellar cryolysis of cornea with amniotic membrane transplantation can relieve the pain in 98.7% of PBK patients and simple therapy for treating PBK. Hence, it's be worth to advocate for relieve the pain of patients.