准分子激光角膜磨镶术后患者泪液中基质金属蛋白酶-2的表达

Expression of matrix metrallproteinase-2 in human tears fluid after LASIK

摘要:

目的：观察成功准分子激光角膜磨镶术(laser-assisted in situ keratomileusis，LASIK)术后人眼泪液中基质金属蛋白酶-2(matrix metalloproteinase-2，MMP-2)的长期变化。

方法：分别收集32例64眼LASIK手术的近视患者术前、术后15d； 1，3mo； 1a的泪液，进行Western-blot实验，检测MMP-2浓度的变化。

结果：LASIK手术导致术后患者泪液中MMP-2浓度增加。术后15d，MMP-2约为术前的1.4倍，随着术后时间的延长逐渐下降，术后3mo仍未恢复(P<0.05)，术后1a几乎恢复至术前水平(P>0.05)。

结论：LASIK手术增加人眼泪液中MMP-2的浓度，且随着术后时间的延长逐渐下降，术后3mo仍未恢复，术后1a恢复至术前水平，表明LASIK诱发的创伤修复反应至少持续3mo，术后1a几乎恢复。

Abstract:

AIM: To monitor long-term changes of matrix metalloproteinase-2(MMP-2)in human tears fluid after laser in situ keratomileusis(LASIK).

METHODS: Thirty-two myopia cases(64 eyes)underwent uneventful LASIK were enrolled in the study. Tear fluid were collected and MMP-2 expression was analyzed by Western-bolt assay preoperatively and postoperatively on 15d, at 1, 3mo, and 1a.

RESULTS: LASIK increased the concentration of MMP-2 in human tear fluid. At 15d postoperatively, the magnitude of MMP-2 was 1.4 times that of preoperative, thereafter subsided, but didn't return to preoperative level by 3mo(P<0.05). Up to 1a after surgery, the concentration of MMP-2 almost recovered(P>0.05).

CONCLUSION: MMP-2 is significantly expressed in human tear fluid after LASIK, then subsided with time, but didn't return to preoperative level by 3mo and almost recovered up to 1a, indicating wound healing of LASIK would continue up at least 3mo after surgery and almost recovered 1a postoperatively.