方法：采用高氧诱导的方法制作鼠视网膜新生血管模型，运用荧光造影视网膜铺片及视网膜切片苏木精-伊红染色观察视网膜新生血管的形态。于小鼠出生后第7，12，14，17，26d取视网膜组织，采用Real-time PCR及Western blot技术测定视网膜组织中Islet-1的表达水平。

结果：模型组视网膜铺片及组织切片可见大量视网膜新生血管形成。小鼠出生后第7d，模型组与正常组视网膜组织中Islet-1表达水平无明显差异； 小鼠出生后第12～14d，模型组视网膜组织中Islet-1表达水平明显上调； 出生后17d，模型组视网膜组织中Islet-1表达水平仍高于正常组； 出生后26d，随着视网膜新生血管消退，视网膜组织中Islet-1表达水平降至正常水平。

结论：模型鼠视网膜新生血管发生过程中，持续缺氧的视网膜组织通过增加转录因子Islet-1的表达，从而诱导视网膜新生血管的发生。

METHODS: The murine retinal neovascularization were induced by hyperoxia exposure.The morphological observation of retinal neovascularization was performed using angiography by fluorescein dextran injection under the fluorescence microscope, and the new blood vessels were quantified after 5d in room air(17-day-old)by counting the vascular epithelial cell nuclei protruding into viteous cavity using HE stain. Realtime PCR and Western blot were used to examine retinal Islet-1 level in postnatal 7,12, 14,17 and 26d respectively.

RESULTS: A lots of new blood vessels were demonstrated in the mouse retina in hyperoxic group by fluorescein angiography and histological method. Moreover, no significant difference was found in retinal Islet-1 level in postnatal 7d between hyperoxic group and control group, but was significantly higher in postnatal 12, 14 and 17d mice compared with control mice. However, mice at postnatal 26d, expression of Islet-1 in retina decreased to normal level.

CONCLUSION: In processing mouse model of retinal neovascularization, sustained hypoxia retinal tissue induce retinal neovascularization by increas the expression of transcription factor Islet-1.