低强度白光照射对小鼠视网膜的影响

Effect of low intensity white light irradiation on the retinas of mice

摘要:

目的：研究低强度白光照射对小鼠视网膜的影响。

方法：C57BL/6J小鼠30只，随机分为两组，每组15只，实验组每天下午4：00～5：00暗适应，5：00～6：00用LED白灯光照，光照强度为100lux，实验时间为1mo。 实验开始第1，3，7，14，30d测视网膜外核层(outer nuclear layer，ONL)厚度，内核层(inner nuclear layer，INL)厚度变化，组织学染色及视觉电生理变化。

结果：实验后1mo，实验组与对照组相比，Rod-R a波潜伏期延长，Cone-R b波振幅下降，b波潜伏期延长，与对照组比较有显著性差异(P<0.05)。实验组与对照组在组织学染色上无明显差异，外核层厚度无明显变化，内核层厚度亦无明显变化。

结论：100lux低强度白光照射可引起暗适应后小鼠视网膜功能发生改变。

Abstract:

AIM: To investigate the effect of low intensity white light irradiation on the retinas of mice.

METHODS: Thirty C57BL/6J mice were randomly divided into two groups. The number of the mice in each group was 15. The mice in experimental group received dark adaptation from 5:00p.m. to 6:00p.m.,and then exposed to LED white light from 6:00p.m. to 7:00p.m. everyday for a month. At 1, 3, 7, 14 and 30d after the beginning, we examed the histology of mice retinas, calculated the thickness of outer nuclear layer(ONL),inner nuclear layer(INL)and analyzed electrophysiology of mice.

RESULTS:One month after experiment, compared to the control group, the latency of Rod-R a wave of the mice in experimental group significantly prolonged, the amplitude of Cone-R b wave of the mice in experimental group significantly decreased and the latency of b wave of the mice in experimental group significantly prolonged(P<0.05).There are no significant difference in the histology of retina, ONL and INL thicknesses.

CONCLUSION: 100lux low intensity white light could give rise to the impairment of the retinal functions in dark-adapted mice.