大豆黄酮玻璃体腔注射对大鼠视神经损伤的影响

Effect of Daidzein intravitreal injection on optic nerve injury in rats

摘要:

目的：大豆黄酮(Daidzein)玻璃体腔内注射是否对视神经急性损伤有保护和修复作用。

方法：SD雄性大鼠72只，建立大鼠急性视神经钳夹损伤模型，随机分为正常组、PBS阴性对照组、大豆黄酮治疗组(10μmol/L，100μmol/L，1 000μmol/L)、鼠神经生长因子(mNGF，100ng/mL)阳性对照组； 玻璃体内注射3d后处死各组大鼠，用组织病理(HE)、免疫组化检测以及Western Blot分析来观察和评价视网膜形态变化及进行各组间视网膜Caspase-3蛋白和GAP-43蛋白表达的比较。

结果：与正常组和阴性对照组相比，不同浓度的大豆黄酮各治疗组和阳性对照组的视网膜形态结构较完整，Caspase-3蛋白的表达相对降低，GAP-43蛋白的表达相对较高，其差异有统计学意义(P<0.05)。

结论：大豆黄酮玻璃体腔注射对大鼠急性视神经损伤具有修复和保护作用。

Abstract:

AIM: To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages.

METHODS:After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control group, Daidzein treatment group(10μmol/L, 100μmol/L, 1000μmol/L)and positive control group using rats nerve growth factor(mNGF, 100ng/mL). Three days after interference, all experimental animals were executed. HE staining was used to evaluate morphologic change of the retina, immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase-3 and GAP-43 among the groups.

RESULTS:Compared with the normal group and negative control group, retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete, the expression of Caspase-3 protein was relatively lower, the expression of GAP-43 protein was relatively higher, the differences have statistically significance(P<0.05).

CONCLUSION: Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat's acute nerve damages.