方法：采用免疫组织化学SP法，检测VEGF、CD34、Ki-67和p21在62例翼状胬肉和对照组20例结膜组织中的表达情况，并分析其与胬肉临床病理特征的关系。

结果：(1)62例翼状胬肉组织中，VEGF、CD34、Ki-67和p21的阳性表达率分别为74.2%(46/62)，77.4%(48/62)，66.1%(41/62)，40.3%(25/62)，与对照组相比，差异均有统计学意义(P<0.05)；(2)在62例翼状胬肉组织中，VEGF、CD34的表达与临床分期及临床类型关系密切(P<0.05)，与患者性别、年龄、职业等无关(均为P>0.05)； Ki-67的表达与临床分期关系密切(P<0.05)，与其它临床病理指标无关(均为P>0.05)； p21的表达与临床分期、胬肉性质关系密切(P<0.05)，与其它临床病理指标无关(均为P>0.05)；(3)VEGF与Ki-67的表达呈正相关(r=0.279，P<0.05)； VEGF与CD34的表达呈正相关(r=0.299，P<0.05)； VEGF与p21表达呈负相关(r=-0.267，P<0.05)； Ki-67与CD34的表达无相关性(r=0.021，P>0.05)； Ki-67与p21的表达无相关性(r=-0.176，P>0.05)； CD34与p21的表达无相关性(r=-0.185，P>0.05)。

结论：(1)VEGF、CD34和Ki-67在翼状胬肉中高表达，p21在翼状胬肉中低表达，提示它们可能参与了翼状胬肉的发生发展过程；(2)VEGF、CD34在翼状胬肉中的阳性表达率随临床分期、临床类型的提高而上升，Ki-67在翼状胬肉中的阳性表达率随临床分期的提高而上升，p21在翼状胬肉的阳性表达率随临床分期、临床类型进展而降低，提示它们可能在翼状胬肉的发生发展及复发过程中发生了作用；(3)VEGF与Ki-67的表达呈正相关，VEGF与CD34的表达呈正相关，VEGF与p21的表达呈负相关，提示两两之间在翼状胬肉发生发展中具有协同作用。

METHODS: Immunohistochemical S-P staining method was adopted in detecting the expression of VEGF, CD34, Ki-67 and p21 in 62 cases of pterygia and 20 cases of normal conjunctival tissues. Relationship between these markers and clinical pathological characteristics was analyzed.

RESULTS:(1)The positive expression of VEGF, CD34, Ki-67 and p21 in 62 cases of pterygia was 74.2%(46/62), 77.4%(48/62), 66.1%(41/62)and 40.3%(25/62)respectively. The differences were statistically significant compared with normal conjunctival tissues(P<0.05).(2)The expression of VEGF and CD34 in 62 cases of pterygia was correlated with clinical types and stages(P<0.05), and was not associated with sex, age and occupation(P>0.05); the expression of Ki-67 was correlated with clinical stages(P<0.05), and was not associated with other clinical pathological characteristics(P>0.05); the expression of p21 was correlated with clinical stages and pterygium characters(P<0.05), and was not associated with other clinical pathological characteristics(P>0.05).(3)Spearman correlation showed that there was a positive correlation between VEGF and Ki-67(r=0.279, P<0.05), a positive correlation between VEGF and CD34(r=0.299, P<0.05), a negative correlation between VEGF and p21(r=-0.267, P<0.05); it also showed that there was no correlation between any two of CD34, Ki-67 and p21(P>0.05).

CONCLUSION:(1)Overexpression of VEGF, Ki-67, CD34 and low expression of p21 suggest that these markers are concerned with the development and progression of pterygium.(2)Expression of VEGF and CD34 increases along with the increase of clinical types and stages, expression of Ki-67 increases along with the increase of clinical stages, and expression of p21 decreases along with the improvement of clinical types or stages; they suggest that these markers may play important roles in the development and recurrence of pterygium.(3)There is positive correlation between VEGF and Ki-67, VEGF and CD34 as well as negative correlation between VEGF and p21. They suggest that there may be synergistic action between two factors during the development and progression of pterygium.