单纯疱疹病毒性角膜炎的实验室检查现状及进展

Progress on the diagnosis of herpes simplex keratitis

摘要:

单纯疱疹病毒性角膜(herpes simplex keratitis，HSK)是一种严重的感染性致盲性眼病。过去HSK的诊断主要依据临床特征，然而这并不可靠。随着分子生物学的发展，目前对于单纯疱疹病毒性角膜炎的诊断有了突飞猛进的发展。共焦显微技术、环媒恒温扩增技术、实时荧光探针定量聚合酶链式反应(polymerase chain reaction， PCR)、巢式PCR(nested PCR)及多重聚合酶链式反应(multiplex PCR)等被认为是较敏感且快速的诊断方法。

Abstract:

Herpes simplex keratitis(HSK)is the leading cause of infectious blindness. In general, diagnosis of HSK is based on clinical findings, which often are not confirmatory. With the development of molecular biology, a variety of laboratory methods that helped in achieving a diagnosis including in vivo confocal microscopy(IVCM), loop-mediated isothermal amplification(LAMP), real-time polymerase chain reaction(rt-PCR), nested PCR and multiplex PCR. They are considered as accurate and fast diagnostic techniques, thus characteristics of these are reviewed in this paper.