方法：临床实验研究。采用流式细胞术检测对Graves病不伴有眼征患者组(53例)，Graves眼病患者组(51例)，正常对照组(51例)的外周静脉血CD4⁺CD25⁺Treg细胞比例进行检测。

结果：与正常对照组比较，Graves病不伴有眼征患者组Treg水平下降(P<0.01)； 与正常对照组比较，Graves眼病患者组外周血Treg水平显著下降(P<0.01)； Graves眼病患者组外周血Treg数量明显低于Graves病不伴有眼征患者组(P<0.01)。

结论：Graves眼病患者外周血中CD4⁺CD25⁺Treg在淋巴细胞中所占的比例降低，存在自身免疫调节紊乱。可能是其机体细胞免疫抑制受损的重要机制，为对该疾病进行免疫学治疗提供了新线索。

METHODS: This experimental study selected 53 GO patients without exophthalmos, 51 GO patients with exophthalmos, and 51 healthy people were collected. The ratio of CD4⁺CD25⁺ T cell in peripheral blood was determined by flow cytometry.

RESULTS: The ratio of CD4⁺CD25⁺ T cell in GO patients without exophthalmos was lower than that in healthy group(P<0.01). The ratio of CD4⁺CD25⁺ T cell in GO patients with exophthalmos was far lower than that in healthy group(P<0.01). Compared with the group of GO patients without exophthalmos, the ratio of CD4⁺ CD25⁺ T cell of GO patients with exophthalmos was also much lower(P<0.01).

CONCLUSION: The ratio of CD4+ CD25+ T cell in the GO patients decreases and there is autoimmune disorder in patients of this disease. Perhaps this is an important mechanism causing immune suppression damage, which provides a new clue for immunological treatment.