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[摘要]
目的： 研究我国视网膜母细胞瘤(retinoblastoma，RB)中HPV-DNA表达情况，为进一步揭示HPV 在散发性RB中作用提供初步依据。
方法：收集22例散发性RB患者新鲜肿瘤组织标本，应用PCR方法及引物MY09/11 检测肿瘤组织中HPV-DNA表达情况。
结果：选取22例RB肿瘤标本中7例HPV-DNA阳性(32%)，均为单眼散发患者(包括男4例，女3例)，2例双眼散发患者均为阴性。
结论： HPV可能是我国散发性RB发病因素之一，但其明确作用及机制尚待进一步研究。
[Key word]
[Abstract]
AIM: To investigate the expression of HPV-DNA in retinoblastoma tumor samples in China, and to provide preliminary evidence for the role of HPV in sporadic retinoblastoma(RB).
METHODS: Fresh tumor tissues from 22 children with sporadic RB were collected to detect the presence of HPV-DNA using polymerase chain reaction(PCR)and MY09/11 primers.
RESULTS: HPV-DNA was present in 7/22(32%)RB tumors. All the positive cases were sporadic unilateral RB(4 cases were male, 3 cases were female). Two bilateral RB cases were negative for HPV-DNA.
CONCLUSION: The presence of HPV in RB tumors can be a pathogenic factor for sporadic RB. But its role and mechanism in carcinogenesis needs further research.
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