方法：SD大鼠40只40眼，通过1mol/L的NaOH建立角膜碱烧伤模型。对照组(A组)行PBS液点眼4次/d，其余三组(B、C、D组)分别行240，400，560μg/mL的AM点眼。通过裂隙灯显微镜记录并比较四组角膜新生血管的生长情况。角膜共聚焦显微镜动态观察损伤后角膜的超微结构。

结果：损伤后的各个时间点，C组的角膜新生血管面积与A、B两组均有显著性差异(P<0.05)，即随着羊膜匀浆提取液浓度的增加，其抑制角膜新生血管的作用增强。但是C、D两组之间的CNV面积差异无统计学意义。角膜共聚焦显微镜观察发现，羊膜匀浆提取液治疗组的多形核细胞浸润明显少于对照组。

结论：羊膜匀浆提取液对碱烧伤后角膜新生血管的抑制作用呈剂量依赖性，但当浓度达到400μg/mL，其对角膜新生血管的抑制作用趋于稳定。这可能部分与羊膜匀浆提取液抑制碱烧伤炎症反应有关。

METHODS: Forty eyes from 40 Sprague-Dawley male rats were used. After alkali injuries at central corneal using 1mol/L NaOH,the control group(Group A)received topical PBS four times a day. The rest groups(Group B, C, D)received topical AM suspension with different concentration(240, 400, 560μg/mL). Using slit lamp biomicroscopy, CNV were evaluated and scored. Tissue ultrastructure changes of cornea were analyzed by corneal confocal microscope.

RESULTS:The area of CNV were statistically different at each time point between group C and group A, B(all P<0.05). CNV were reduced as AM concentrations increased, while there were no significant differences between group C and group D. Inflammatory cells in corneal were reduced in group AM-treated groups.

CONCLUSION: AM suspension leads to significant reduce in CNV with increasing AM concentrations. However, when the concentration reached 400μg/mL, the effects on restraining CNV cannot be enhanced by concentration. This effect may be elicited in part through the inhibiting of inflammatory cells.