方法：选取年龄40～70岁、糖尿病病程10～20a、血压<140/90mmHg且不合并糖尿病肾病的2型糖尿病患者90例，其中无DR组(NDR组)、非增生型DR组(BDR组)和增生型DR组(PDR组)各30例，采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术，对所有患者PPAR-γ2基因Pro12Ala多态性位点进行基因分型。

结果：该研究人群中存在2种等位基因和3种基因型，其中NDR组中基因型PP，PA，AA分别为40.0%，53.3%和6.7%，BDR组分别为70.0%，30.0%和0%，PDR组分别为76.7%，23.3%和0%； 三组间比较，基因型频率(χ²=10.351)和等位基因频率(χ²=10.208)的差异均有统计学意义(P< 0.05)。Logistic回归分析结果显示Pro12 Ala基因多态性是DR发生的危险因素。

结论：PPAR-γ2基因Pro12Ala多态性与山西地区部分汉族人群2型糖尿病视网膜病变有关，且可能是抑制其发生的保护性因子。

METHODS: Totally 90 patients with T2DM were selected into our research, who were at the age of 40 to 70 years old, diabetic duration from 10 to 20 years, blood pressure <140/90mmHg, and without diabetic nephropathy. Thirty persons were respectively divided into non-DR(NDR)group, nonproliferative-DR(BDR)group and proliferative-DR(PDR)group according to the result of ophthalmology examination. The genotypes of Pro12Ala polymorphism of PPAR-γ2 gene were determined by polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP)assay in all the patients.

RESULTS: PCR results showed that there were 2 alleles and 3 genotypes in the groups. The frequency of genotype PP, PA, AA were 40.0%, 53.3%, 6.7% in NDR group, 70.0%, 30.0%, 0.0% in BDR group, 76.7%, 23.3%, 0% in PDR group, respectively. The allele frequency(χ²=10.208)and gene frequency(χ²=10.351)were statistically significant(P<0.05)in these groups. Logistic regression analysis showed that Prol2Ala polymorphism was a dangerous factor in the incidence of DR.

CONCLUSION: The alanine variant of Prol2Ala polymorphism of PPAR-γ2 gene is associated with DR in type 2 diabetes among the Hans in Shanxi area, and the Ala allele might be a protective factor for the development of diabetic retinopathy.