方法：利用美国国立图书馆的PubMed数据库为数据源，对2000/2012年所收录的角膜移植手术相关文献的年代分布、国家和地区、发文语种及作者情况等进行统计分析，并利用书目共现分析系统(BICOMB)、SPSS 19.0分析软件对高频主题词进行聚类分析。

结果：2000/2012年累计收录角膜移植手术相关研究文献3 363篇，文献数据逐渐增多； 研究文献以英语为主，我国文献量位列第4； 统计出高频主题词50个，聚类分析显示高频主题词主要聚类于3个类别，分别为角膜内皮移植手术、角膜移植手术后并发症治疗、角膜移植手术后的免疫排斥反应。

结论：角膜移植手术研究文献呈增长趋势，研究热点为角膜内皮移植手术。

METHODS: Articles were searched from PubMed of the United States national library database as a data source, to analyze the age distribution, national and regional distribution, dispatch language and author situation included during the year of 2000-2012. The analysis software BICOMB and SPSS 19.0 were used to cluster high-frequency of them.

RESULTS: Totally 3 363 literatures were included during the year of 2000-2012, the numbers of them were gradually increased. The literatures were mainly in English, the literatures of our country capacity ranked 4^th. There were 50 high frequency subjects, and hot topics were clustered into 3 categories which including corneal endothelium transplantation, treatment for the complications of corneal transplantation, immunological rejection of corneal transplantation.

CONCLUSION:There is a growing trend on the research of corneal transplantation. The corneal endothelium transplantation is the hot topics.