[关键词]
[摘要]
目的：研究缺氧诱导因子-1α(hypoxia-inducible factor-1α，HIF-1α)及其靶基因血管内皮生长因子(vascular endothelial growth factor，VEGF)在增殖型性糖尿病视网膜病变(proliferative diabetic retinopathy，PDR)视网膜前膜的表达，探讨其在PDR发生发展中的作用。
方法：针对26例PDR Ⅴ～Ⅵ期患者，行玻璃体切除+剥膜术(有合并视网膜脱离者加行视网膜复位术)，术中取出视网膜前膜行免疫组织化学染色，观察视网膜前膜中HIT-1α与VEGF和血管内皮标志物CD34的表达。同时行术前血糖、甘油三脂、糖化血红蛋白、是否合并视网脱进行分类并行多因素Logistic回归分析。
结果：PDRⅤ～Ⅵ期视网膜前膜血管内皮细胞中，表达HIT-1α者24例(92.3%)，VEGF者15例(57.7%)，血管内皮标志物CD34者26例(100%)。血管内皮标志物CD34分别与HIT-1α(r=0.556，P=0.028)和VEGF(r=0.745，P=0.001)直线相关。 术前血糖、甘油三脂、糖化血红蛋白、是否合并视网脱是PDR视网膜前膜中表达因子HIT-1α，VEGF和CD34表达的相关因素。
结论：HIT-1α和VEGF在糖尿病视网膜病变视网膜前膜中明显表达。HIT-1α和VEGF可能在PDR的发病中起重要作用。
[Key word]
[Abstract]
AIM:To investigate the expression of the hypoxia-inducible factor-1α(HIF-1α)and the protein products of its target gene vascular endothelial growth factor(VEGF)in proliferative diabetic retinopathy(PDR)epiretinal membranes.
METHODS: Totally 26 patients who have been obtained epiretinal membranes(PDRⅤ-Ⅵ)were performed pars- plana vitrectomy. They were removed the epiretinal membrane intraoperation and to observe HIF-1α, VEGF and expression of vascular endothelial marker CD34 in epiretinal membranes through immunohistochemical method.
RESULTS:Vascular endothelial cells of PDR expressed HIF-1α in 24 cases(92.3%)and VEGF in 15 cases(57.7%)in membranes, respectively. There were significant correlations between the numbers of blood vessels expressing CD34 and HIF-1α(r=0.556, P=0.028). There were significant correlations between the numbers of blood vessels expressing CD34 and VEGF(r=0.745,P=0.001).
CONCLUSION:HIF-1α and VEGF may play an important role in the pathogenesis of PDR.
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