目的：观察补肾活血中药对大鼠慢性高眼压(elevated intraocular pressure，EIOP)模型初级视皮质(primary visual cortex，PVC)脑源性神经营养因子(brain-derived neurotrophic factor，BDNF)的干预作用，并对其作用机理进行初步探讨。

方法：采用烙闭上巩膜静脉法，烙闭大鼠3支上巩膜静脉，制作大鼠慢性EIOP模型，随机分为3组：模型组，给药组，空白组。连续灌胃8wk，并于8wk末处死大鼠，观察其对EIOP大鼠眼压、PVC的BDNF表达及神经元细胞超微结构的影响。

结果：本实验采用的烙闭上巩膜静脉的造模方法使大鼠眼压明显升高(P<0.01)，与造模前比较差异有显著统计学意义(P<0.01)； PVC病理切片半定量分析表明，模型组BDNF总面积82438±2597.39(S/μm²)、平均光密度1155.9±123.14、积分光密度12915±673.28，与空白组(总面积132370±7588.47S/μm²，平均光密度5365±379.65，积分光密度35102±2648.5)比较均有显著统计学意义(均为P<0.05)，模型组BDNF总面积与给药组(108980±9126.77S/μm²)比较有统计学意义(P<0.05)，给药组平均光密度(3220.4±413.67)与模型组比较有统计学意义(P<0.05)，给药组积分光密度(23821±3431.68)与模型组比较，差异有统计学意义(P<0.05)。

结论：补肾活血中药通过增强BDNF的表达、改善神经元细胞超微结构而促进EIOP大鼠初级视皮质损伤的修复。

AIM: To observe the effect of traditional Chinese medicine(TCM)of bushenhuoxue on primary visual cortex(PVC)brain-derived neurotrophic factor(BDNF)in rat model of chronic elevated intraocular pressure(EIOP), and explore the mechanism of it initially.

METHODS: The rat model of chronic EIOP was established by unilaterally cauterizing 3 episcleral veins, then 30 rats were divided into 3 groups randomly: control group, model group, and treatment group. After given drugs or normal saline for 8 weeks, the rats were put to death. The effect of intraocular pressure(IOP), expression of BDNF and ultrastructure of neuron cell in the PVC was observed.

RESULTS: Unilaterally cauterizing episcleral veins increased IOP of the rat model obviously, there was significant difference compared with pre-operation(P<0.01). Semi-quantitative pathological analysis on PVC showed that BDNF of total area in the model group was(82438±2597.39)S/μm²,mean optical density was(1155.9±123.14), integrated optical density was(12915±673.28), compared with the control group {total area was(132370±7588.47)S/μm², mean optical density was(5365±379.65), integrated optical density was(35102±2648.5)}, there were statistical differences(all P<0.05),there was statistical difference in BDNF of total area between model group and treatment group{(108980±9126.77)S/μm², P<0.05}, significant difference in mean optical density between the model group and treatment group(3220.4±413.67, P<0.05), statistical difference in integrated optical density between the model group and treatment group(23821±3431.68, P<0.05).

CONCLUSION: TCM of bushenhuoxue can repair the PVC damage in the rat model of chronic EIOP by enhancing expression of BDNF, improving ultrastructure of neuron cell.

中国四川省教育厅2010年重点项目(No.10ZA096)