方法：铜绿假单胞菌生物膜中加入不同浓度的游离铁、不同浓度及不同种类的合成铁和螯合铁条件下定量检测生物膜上的菌落数。

结果：低浓度游离铁离子的作用可以增强细菌的黏附量， 以10μmol/L铁离子的作用最明显。0.1μmol/L EDTA和乳铁蛋白可明显抑制铜绿假单胞菌生物膜的菌数。

结论：低浓度游离铁离子的作用可以促进隐形眼镜铜绿假单胞菌生物膜形成，而一定浓度的乳铁蛋白及EDTA形成的缺铁情况下可阻止该生物膜的形成。

METHODS:Different concentration of free iron, different concentration and variety classes of synthesis iron and biological iron chelating agent were added into pseudomonas aeruginosa biofilm, and then the clump count on the biofilm was detected.

RESULTS:Low concentration of free iron increased the adhesive capacity of the bacterial, with 10μmol/L of iron had the most obvious effect. 0.1μmol/L of EDTA and lactoferrin significantly inhibited the bacterial count of pseudomonas aeruginosa biofilm.

CONCLUSION: Low concentration of free iron can promote the bacterial count of pseudomonas aeruginosa biofilm on the contact lens, the lack iron circumstance which formed by finite concentration lactoferrin and EDTA can prevent the formation of the biofilm.