Caspase-3在形觉剥夺性弱视大鼠大脑视皮层的表达

Expressions of caspase-3 in visual cortex of form deprivation amblyopia rats

摘要:

目的：检测凋亡相关因子半胱氨酸天冬氨酸蛋白酶(Caspase-3)在形觉剥夺性弱视大鼠大脑视皮层中的表达及意义。

方法：建立形觉剥夺弱视大鼠模型，对10只正常大鼠和10只单眼形觉剥夺弱视大鼠大脑视皮层17区进行HE染色观察形态学变化，采用免疫组化法以及图像分析系统对Caspase-3免疫阳性神经元进行定位观察并定量研究其变化。

结果：正常大鼠和单眼形觉剥夺弱视大鼠视皮层17区各层次均可见Caspase-3免疫阳性神经元存在，而以Ⅱ~Ⅳ层较多。与正常大鼠相比，Caspase-3在单眼剥夺性弱视大鼠组视皮层17区Ⅱ～Ⅳ层的表达比正常组明显增多，差异具有统计学意义(P<0.05)。

结论：Caspase-3在单眼剥夺弱视大鼠大脑视皮层17区表达升高，可能参与弱视的发生、发展过程。

Abstract:

AIM: To investigate the expressions and significance of apoptosis relevant factor caspase-3 in visual cortex area 17 of form deprivation amblyopia rats.

METHODS: Form deprivation amblyopia rats models were established, the morphological changes of visual cortex area 17 in 10 normal rats and 10 form deprivation amblyopia rats were stained with HE and observed HE staining, processed by immunohistochemical staining and image analysis system to demonstrate and analyze the localization of caspase-3 in area 17.

RESULTS: At various levels of the form deprivation amblyopia rats and normal rats, there were lots of caspase-3 immunoreactive neurons in visual cortex area 17, especially Ⅱ-Ⅳlayer. The number and grey level of caspase-3 immunopositivity increased greatly than normal rats(P<0.05).

CONCLUSION: Caspase-3 in visual cortex area 17 of form deprivation amblyopia rats has increased expression, which may be involved in the development of amblyopia.