雌激素对缺氧视网膜Müller细胞PEDF和VEGF表达的影响

Effect of estrogen on the expression of PEDF and VEGF in cultured retinal Müller cells under hypoxic condition

摘要:

目的：探讨雌激素对缺氧视网膜Müller细胞色素上皮衍生因子(PEDF)和血管内皮生长因子(VEGF)表达的影响。

方法：采用反转录聚合酶链反应(RT-PCR)和Western-blot印迹分析方法分别测定不同浓度雌二醇(E₂)作用于缺氧Müller细胞后，细胞内PEDF mRNA，VEGF mRNA及相应的蛋白表达水平。

结果：缺氧24h后PEDF mRNA及蛋白表达明显降低，10^-5mmol/L和10^-6mmol/L E₂作用于Müller细胞后可明显缓解由于缺氧引起的细胞内PEDF mRNA及蛋白表达的降低，并与E₂的浓度有关。缺氧24h后VEGF mRNA及蛋白表达明显升高，10^-5mmol/L和10^-6mmol/L E₂作用于Müller细胞后可以明显降低细胞内VEGF mRNA及蛋白表达水平，并与E₂的浓度有关。

结论：雌激素可以调控缺氧条件下视网膜Müller细胞内PEDF和VEGF的表达，对视网膜病理性新生血管的形成具有保护作用。

Abstract:

AIM: To explore the effects of estrogen on the expression of pigment epithelium derived factor(PEDF)and vascular endothelial growth factor(VEGF)in the cultured rat retinal Müller cells under hypoxia.

METHODS: Effects of estrogen on the expression of PEDF and VEGF in the cultured rat retinal Müller cells were investigated with the methods of RT-PCR and Western blotting analysis.

RESULTS: The levels of PEDF mRNA and protein decreased and VEGF mRNA and protein increased when the Müller cells were under hypoxic condition for 24 hours. Estrogen had protective effect on Müller cells.

CONCLUSION: Estrogen can regulate the expression of PEDF which may play an important role on retinal neovascularization.