方法：观察在本院行Epi-lasik手术的患者112眼，所有病例随机分为A，B两组： A组(小牛血去蛋白提取物组)58眼，B组(重组人表皮生长因子衍生物组)54眼，两组分别在Epi-lasik术后，立即用小牛血去蛋白提取物和重组人表皮生长因子衍生物眼药水，并观察患者术后刺激症状、术后角膜上皮瓣生长情况、术后视力及达到术前最佳矫正视力的时间、haze等情况，随访6～12mo。

结果：A组的角膜上皮瓣生长情况要好于B组，两组间的差异有统计学意义(P<0.05)。术后1mo检查，A组有45眼(77.6%)达到术前最佳矫正视力，B组有32眼(59.3%)，两组差异有统计学意义(P<0.05)。在达到术前最佳矫正视力的时间上，A组的大部分患者在术后1wk以内达到，而B组的患者多在术后2wk以后达到，两组差异有统计学意义(P<0.05)。两组在对术后haze的影响方面没有显著性差异(P>0.05)。

结论：在Epi-lasik手术中应用小牛血去蛋白提取物，可以减轻角膜上皮瓣的水肿，缩短术后视力恢复的时间，改善干眼症状，安全有效，具有良好的应用前景。

METHODS: Totally 112 eyes with Epi-LASIK were randomly divided into Group A(protein-free calf blood extract eye gels)and Group B(recombinant human epidermal growth factor, rhEGF): Group A with 29 patients 58 eyes were treated by protein-free calf blood extract eye gels and Group B with 27 patients 54 eyes were treated by rhEGF eye drops. In each group after Epi-LASIK, the drug was used immediately. Postoperative irritation, growth of epithelial flap, postoperative visual acuity, time to achieve the preoperative best-corrected visual acuity, haze development etc were observed for 6-12 months.

RESULTS: The growth of epithelial flap in the Group A was better than the Group B and there was statistically significant difference(P<0.05). One month after surgery, 45 eyes(77.6%)in the Group A and 32 eyes(59.3%)in the Group B achieved the preoperative best-corrected visual acuity which was statistically significant(P<0.05). The time to achieve the preoperative best-corrected visual acuity in most Group A patients was shorter than 1 week while in the Group B it was longer than 2 weeks, which was statistically significant(P<0.05). There was no significant difference in haze development between 2 groups(P>0.05).

CONCLUSION: Application of protein-free calf blood extract after Epi-LASIK can relieve epithelial flap edema, shorten time of visual acuity rehabilitation and alleviate the sensation of dry eye. It is a safe, effective method and presents a captivating prospect.