方法：用CFA+PTX+IRBP对6～10周龄雌性C57BL/6小鼠后肢及尾部皮下进行三点免疫建立EAU模型，于免疫3，7，14，21，28d取外周血行流式细胞术检测。

结果：用特异性抗原IRBP免疫后，EAU疾病在第14d左右产生，在第21d达最高峰，以后开始逐渐缓解。随着EAU疾病的发生，CD4⁺CD25⁺调节性T细胞、CD4⁺CD3⁺辅助T细胞数量均有增加，CD4⁺CD25⁺调节性T细胞增加更为明显，CD4⁺CD25⁺Foxp3⁺调节性T细胞数量第21d达到高峰，第28d开始下降，CD4⁺CD25⁺Foxp3⁺/CD4⁺CD25^-Foxp3⁺比值从第3d开始逐渐增加，到第21d达到高峰，从第28d开始下降。

结论：EAU疾病的发生和转归与CD4⁺CD25^+/-Foxp3⁺Treg细胞密切相关，CD4⁺CD25^+/-Foxp3⁺Treg细胞为阐明EAU的缓解机制、预防和治疗人类葡萄膜炎提供了新思路。

METHODS: EAU was induced in C57BL/6 mice by subcutaneous immunization of CFA+PTX+IRBP in hind limb and empennage. Peripheral blood flowcytometry was performed at 3, 7, 14, 21 and 28 days after immunization, respectively.

RESULTS: After immunization with specific antigen IRBP, clinical assessment showed EAU occurred at 14 days and became severe at 21 days, but gradually relieved at 28 days. Accompanying with intraocular inflammation of EAU, both CD4⁺CD25⁺ T regular cells and CD4⁺CD3⁺ helper T cells increased. Especially,CD4⁺CD25⁺ T regular cells increased more obviously at 21 days. The number of CD4⁺CD25⁺Foxp3⁺ T regular cells had a great raising and gradually reduction after 28 days. The ratio of CD4⁺CD25⁺Foxp3⁺/CD4⁺CD25^-Foxp3⁺ had a piecemeal increase after 3 days and reached the peak at 21 days then reduced at 28 days.

CONCLUSION: The groups of CD4⁺CD25^+/- Foxp3⁺ T regular cells have a close relationship with the development and turnover of EAU. The groups of CD4⁺CD25^+/-Foxp3⁺ T regular cells provide a new thread for clarity remission mechanism of EAU, prevention and treatment of EAU.