PEDF对氧化应激损伤的RGC-5细胞的保护作用

Neuroprotective effects of PEDF against oxidative stress-induced injury in RGC-5cells

摘要:

目的:研究色素上皮衍生因子(PEDF)对分化的视网膜神经节细胞(RGC-5)氧化应激损伤的防护作用。方法:利用外源性活性氧(H₂O₂)造成分化的RGC-5细胞的氧化应激损伤,同时应用PEDF进行保护。用MTT检测其细胞活性,用Annexin V-FITC凋亡试剂盒测定凋亡。结果:H₂O₂以浓度依赖的方式降低RGC-5细胞活性,200μmoL/L H₂O₂在24h可以导致分化的RGC-5细胞活性降低40%；细胞凋亡百分数（36.60%±2.12%）较空白对照组（6.03%±1.45%）明显增加(P<0.01)。100ng/mL PEDF使H₂O₂损伤分化后RGC-5细胞的细胞活性提高了30%,凋亡率降低为（9.36±1.61）%。结论:PEDF能够有效地防护氧化应激对视网膜神经节细胞的损伤,是一种极具开发潜力的神经保护性药物。

Abstract:

AIM:To investigate the neuroprotective effects of pigment epithelium-derived factor(PEDF) against oxidative stress-induced injury in differentiated retinal ganglion cell (RGC)-5 cells.METHODS:After differentiated with staurosporine, RGC-5 cells were divided into 4 groups. After every group cells were cultured for 24 hours, cell viability was determined by MTT assay; the apoptosis percent was tested by the Annexin V-FITC apoptosis kit.RESULTS:H₂O.2 may induce cell death of RGC-5 cells in a concentration-dependant manner. Exposure to 200μmol/L H₂O.2 clearly induced cell death in 40% of the cells within 24 hours. The apoptosis percent (36.60%±2.12%) after exposure to 200μmol/L H₂O₂ is higher than the control cells(6.03%±1.45%). PEDF at 100ng/mL suppressed the cell death induced by H₂O.2about 30% of the control cells. At the same concentration, PEDF also inhibited the cell apoptosis induced by H₂O₂(P<0.01).CONCLUSION:PEDF is able to effectively protect RGC against oxidative stressed injury and can be used as a very potential neuroprotective drug.