结膜印迹细胞学检查在干眼症诊断中的特异性研究

Specificity research of conjunctival blotting cytological examination in diagnosis of dry eye syndrome

摘要:

目的:讨论结膜印迹细胞学检查在干眼症诊断中的特异性。方法:选择干眼症患者45例90眼,正常人15例30眼进行结膜印迹细胞学检查及PAS染色,观察各组结膜上皮细胞及杯状细胞形态数量的变化。结果:正常对照组表现为Nelson分级的0级,轻度干眼症患者表现为Nelson 1级,中度干眼症患者表现为Nelson 2级,重度干眼症患者表现为Nelson 3级。杯状细胞数量,轻度干眼症组111.72±13.63/mm²,中度干眼症组53.66±6.15/mm²,重度干眼症组10.18±3.2/mm²,各组间比较差异具有统计学意义（P＜0.01）。结论:结膜印迹细胞学检查结果客观精确,在干眼症诊断中具有特异性,值得临床推广应用。

Abstract:

AIM:To study the specificity of conjunctival blotting cytological examination in diagnosis of dry eye syndrome.METHODS:Totally 45 cases (90 eyes) with dry eye syndrome and 15 normal volunteers (30 eyes) were applied with conjunctival blotting cytological examination combined PAS staining. The number, morphology and change of conjunctival epithelial cells and goblet cells were observed．RESULTS:The normal volunteers presented the Nelson grade 0, the mild dry eye patients presented the Nelson grade 1, the moderate dry eye patients presented the Nelson grade 2, the severe dry eye patients presented the Nelson grade 3. The number of goblet cells was calculated, there was significant decrease in dry eye (mild:111.72±13.63/mm², moderate:53.66±6.15/mm², severe:10.18±3.2/mm²). The difference was statistically significant in the comparison of each groups（P<0.01）.CONCLUSION:The result of conjunctival blotting cytological examination is accurate and objective in diagnosis of dry eye syndrome. The method is worthy of promoting and applying.