目的：研究基质金属蛋白酶-9 (matrix metalloproteinase-9,MMP-9 )和核转录因子-κB（nuclear factor of κB, NF-κB）在视网膜母细胞瘤(Retinoblastoma, RB)中的表达及与RB分化程度和视神经浸润的关系,探讨它们在RB浸润、转移过程中的作用。方法：应用MMP-9和NF-κB单克隆抗体,采用免疫组织化学方法,对47例RB患者石蜡包埋标本进行MMP-9和NF-κB表达的测定,数据进行统计学分析。结果： （1）47例RB中MMP-9阳性表达率为46.8%, 与RB的分化程度(P<0.05)及有无视神经浸润(P<0.05)相关。（2）NF-κB在RB组织中阳性表达率为63.8%,与RB的分化程度相关(P<0.05),与有无视神经浸润无相关性(P>0.05)。（3）RB中NF-κB的表达与 MMP-9的表达有相关性(P<0.05)。结论：RB组织中MMP-9高表达。 NF-κB在RB中被激活。RB中NF-κB表达与MMP-9表达相关,NF-κB激活后可能通过上调MMP-9的表达促进RB的浸润、转移。二者表达的检测可以作为反映RB浸润、转移潜能的生物学指标,有助于筛选转移高危病例。

AIM: To investigate the expression of matrix metalloproteinase-9(MMP-9) and nuclear factor of κB (NF-κB) in retinoblastoma (RB) and their relationships with differentiation and optic never infiltration for discussion their effect in invasion and metastasis of RB.METHODS:Immunohistochemical technique was adopted to examine the protein expression of MMP-9 and NF-κB in 47 cases. All the data were analyzed by statistics. RESULTS:The expression ratio of MMP-9 was 46.8%, and the expression was also significantly related to the differentiation degree(P<0.05) and never infiltration(P<005). The positive rate of NF-κB expression was 63.8%. The expression had notable relationship with the differentiation degree (P<0.05), but not correlated with the never infiltration (P>0.05). The expression of NF-κB had an obvious relationship with the expression of MMP-9 (P<0.05).CONCLUSION: In RB, MMP-9 expressed in higher level. NF-κB was activated and had higher expression in RB. The expression of NF-κB was related to the expression of MMP-9. The activation of NF-κB can possibly promote invasion and metastasis of RB by up-regulating the expression of MMP-9. Detecting the expression of MMP-9 and NF-κB may be biological indicator of invasion and metastasis of RB and can help to filter cases with high risk of metastasis. 